Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/2169-v
•
11:44 min
September 26th, 2010
Chapters
0:00
Title
1:05
Introduction
2:28
Harvestng Xenographs from Mice
3:22
Generation of a Single-cell Suspension from Xenografts
4:12
Removal of Tissue Debris and Dead Cells
5:44
Staining Cells for Fluorescence Activated Cell Sorting (FACS)
7:43
Isolation of Cancer Stem Cells by FACS
10:15
Conclusion
癌症干细胞(CSCS)已确定在一些恶性肿瘤。在这个协议中,我们描述了一个流式细胞仪方法,利用醛脱氢酶活性和CD44和CD24的表达,从人胰腺癌移植瘤的社区体育会隔离。然后可以使用这些活细胞功能和分析研究。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved