JoVE Logo
Faculty Resource Center

Sign In

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding


Using an anatomical needle, scrape three 2- to 3-centimeter-long wounds in the main veins of detached rice leaves. Place the scraped leaves on toothpicks, and spray them with Tween-20 solution. Next, cut a 1/2- by 1/2-centimeter mycelial plug from the OTA plates containing each M griseus strain. Place the plugs on the wounded leaves, and incubate them in a humid chamber at 25 degrees Celsius for 3 to 8 days.

After the incubation period, examine and evaluate the lesions according to the scoring system provided by the International Rice Research Institute. Finally, photograph the diseased leaves to evaluate the infectiousness of the tested strains.

Usage Statistics

-- Views

Related Videos


Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae


Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone


Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

JoVE Logo


Terms of Use





Copyright © 2024 MyJoVE Corporation. All rights reserved