Name: intravital microscopy of the mouse brain microcirculation using a closed cranial window
Uploaded: 2010-11-18T18:00:00
Description: Watch this Scientific Journal Video about Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window at JoVE.com

这项工作是支持R01 - HL87290补助，R01 - HL87290 - S1和美国国立卫生研究院R01 - AI082610 LJMC。

1。开颅手术需要提前1如前所述，除了一个钛棒是不是动物的头放在一个开颅手术在8到10周大的老鼠。慢性颅窗口是一个稳定的准备允许甚至几个月后，被植入的软脑膜微循环检查。通常情况下，我们执行2-3周后颅窗口植入我们的研究。 2。活体显微镜<ol><li>开颅手术后两三个星期，体温检查，然后鼠标轻轻麻醉与异氟醚（归纳为4％，维持1-2％）...

<ol>
	<li>Mostany, R., Portera-Cailliau, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+craniotomy+surgery+procedure+for+chronic+brain+imaging.">A craniotomy surgery procedure for chronic brain imaging.</a> J. Vis. Exp. (15), (2008).</li><li>Intaglietta, M., Tompkins, W. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microvascular+measurements+by+video+image+shearing+and+splitting.">Microvascular measurements by video image shearing and splitting.</a> Microvasc. Res. 5, 309-312 (1973).</li><li>Briceno, J. C., Cabrales, P., Tsai, A. G., Intaglietta, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radial+displacement+of+red+blood+cells+during+hemodilution+and+the+effect+on+arteriolar+oxygen+profile.">Radial displacement of red blood cells during hemodilution and the effect on arteriolar oxygen profile.</a> Am. J. Physiol. Heart Circ. Physiol. 286, 1223-1228 (2004).</li><li>Bishop, J. J., Nance, P. R., Popel, A. S., Intaglietta, M., Johnson, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+erythrocyte+aggregate+size+and+flow+rate+in+skeletal+muscle+venules.">Relationship between erythrocyte aggregate size and flow rate in skeletal muscle venules.</a> Am. J. Physiol. Heart Circ. Physiol. 286, H113-120 (2004).</li><li>Baldwin, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+hemoglobins+produce+venular+interendothelial+gaps+and+albumin+leakage+in+the+rat+mesentery.">Modified hemoglobins produce venular interendothelial gaps and albumin leakage in the rat mesentery.</a> Am. J. Physiol. Heart Circ. Physiol. 277, H650-H659 (1999).</li><li>Cabrales, P., Zanini, G. M., Meays, D., Frangos, J. A., Carvalho, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+cerebral+malaria+is+associated+with+a+vasospasm-like+microcirculatory+dysfunction,+and+survival+upon+rescue+treatment+is+markedly+increased+by+nimodipine.">Murine cerebral malaria is associated with a vasospasm-like microcirculatory dysfunction, and survival upon rescue treatment is markedly increased by nimodipine.</a> Am. J. Pathol. 176 (3), 1306-1315 (2010).</li></ol>

这里描述的活体显微镜方法，提供了一个在小鼠软脑膜微循环的详细观察独特而强大的工具。它允许挑出个别动脉和静脉和测量的一些参数如血管直径，红细胞速度，血流量，坚持和白细胞，血小板和其他血液成分，血管渗漏，组织pH值及PO2滚动的变化和潜在的许多其他应用。 在体内的血管反应，可及时进行评估后，如药品监督管理局的干预，或在病理过程。此外，微循环的行为，可以动?...

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<td>Isoflurane</td>
<td>&nbsp;</td>
<td>Baxter</td>
<td>FDG9623</td>
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<td>Carbocyanine dye Dil </td>
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<td>Molecular Probes</td>
<td>D306</td>
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<td>Albumin-FITC</td>
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<td>Sigma-Aldrich</td>
<td>A9771</td>
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<td>Anti-CD45-TxR Ab</td>
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<td>Invitrogen</td>
<td>MCD4517</td>
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intravital microscopy of the mouse brain microcirculation using a closed cranial window

这一实验模型的目的是评估在急性和慢性，生理和病理的血 ​​流动力学，炎症和代谢的条件下小鼠软脑膜微循环 ，在体内荧光显微镜使用。左parieto枕的老鼠的大脑皮层放置在一个封闭的颅窗口。通过使用EPI和荧光照明，测量血管直径和红细胞（RBC）的执行速度的窗口局部微循环是实时记录。使用实时交叉相关性和/或荧光标记的红细胞，红细胞流速测量。白细胞和血小板粘附到软脑膜血管灌注和血管渗漏的评估与荧光标记的标记，如白蛋白- FITC和抗CD45 - TxR还抗体帮助。可以反复录像在几天微。我们用于第一次关闭窗口脑活体显微镜研究软脑膜微循环的动态变化，并跟随在安卡疟原虫感染小鼠当然，显示CM的表达与微循环功能障碍的特点是由血管收缩，减少血液中的深刻流，并最终血管崩溃。

Watch this Scientific Journal Video about Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window at JoVE.com

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

活体显微镜，按照时间和空间在软脑膜微循环血流动力学和炎症事件。

活体鼠脑微循环显微镜使用一个封闭的颅窗口

This experimental model was designed to assess the mouse pial microcirculation during acute and chronic, physiological and pathophysiological hemodynamic, ...

Research

JoVE Journal

Neuroscience

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

制备小鼠脑组织中的免疫电镜

Transmission electron microscopy (TEM) is extremely useful for visualizing microglial, oligodendrocytic, astrocytic, and neuronal subcellular compartments ...

科研

神经科学

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

抛光和钢筋，颅骨变薄窗口，为长期的小鼠脑成像

In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form ...

Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

多光子显微镜的清除小鼠脑组织表达YFP

Multiphoton microscopy of intrinsic fluorescence and second harmonic generation (SHG) of whole mouse organs is made possible by optically clearing the ...

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear

灭绝培训在彭兰霞窗口无法还原的恐惧

Fear is maladaptive when it persists long after circumstances have become safe. It is therefore crucial to develop an approach that persistently prevents ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

小脑颗粒神经元的遗传操作<em&gt;体外</em&gt;和<em&gt;在体内</em&gt;为研究神经元形态和迁移

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

嗅觉系统作为模型来研究轴突生长的模式和形态<em&gt;在体内</em

The olfactory system has the unusual capacity to generate new neurons throughout the lifetime of an organism. Olfactory stem cells in the basal portion of ...

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

方法对敌人造成闭合性颅脑创伤性脑损伤<em&gt;果蝇</em

Traumatic brain injury (TBI) affects millions of people each year, causing impairment of physical, cognitive, and behavioral functions and death. Studies ...

Piezo High Accuracy Surgical Osteal Removal (PHASOR): A Technique for Improved Cranial Window Surgery in Mice

Piezo High Accuracy Surgical Osteal Removal PHASOR: A Technique for Improved Cranial Window Surgery in Mice

压电高精度手术骨切除 (相量): 改进小鼠颅骨窗手术的技术

Multiphoton microscopy has been widely adapted for imaging neurons in vivo. Repeated imaging requires implantation of a cranial window or repeated ...

Laminectomy and Spinal Cord Window Implantation in the Mouse

拉米内切手术和脊髓窗口植入小鼠

This protocol describes a method for spinal cord laminectomy and glass window implantation for in vivo imaging of the mouse spinal cord. An integrated ...

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

免疫荧光定量小鼠脑突触蛋白的异种分布

The presence, absence, or levels of specific synaptic proteins can severely influence synaptic transmission. In addition to elucidating the function of a ...