Name: intravital microscopy of the mouse brain microcirculation using a closed cranial window
Uploaded: 2010-11-18T18:00:00
Description: Watch this Scientific Journal Video about Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window at JoVE.com

この作品は、LJMCに国立衛生研究所からの補助金R01 - HL87290、R01 - HL87290 - S1とR01 - AI082610によってサポートされていました。

1。開頭術 8〜10週齢のマウスの開頭術では、チタンのバーは、動物の頭部に配置されていないことを除いて、前述の1として事前に実行する必要があります。慢性頭蓋ウィンドウが注入された後、軟膜微小循環にも数ヶ月の検査を可能にする安定な製剤である。通常、我々は2〜3週間頭蓋窓の移植後に研究を行う。 2。生体顕微鏡検査<ol><li>開?...

<ol>
	<li>Mostany, R., Portera-Cailliau, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+craniotomy+surgery+procedure+for+chronic+brain+imaging.">A craniotomy surgery procedure for chronic brain imaging.</a> J. Vis. Exp. (15), (2008).</li><li>Intaglietta, M., Tompkins, W. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microvascular+measurements+by+video+image+shearing+and+splitting.">Microvascular measurements by video image shearing and splitting.</a> Microvasc. Res. 5, 309-312 (1973).</li><li>Briceno, J. C., Cabrales, P., Tsai, A. G., Intaglietta, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radial+displacement+of+red+blood+cells+during+hemodilution+and+the+effect+on+arteriolar+oxygen+profile.">Radial displacement of red blood cells during hemodilution and the effect on arteriolar oxygen profile.</a> Am. J. Physiol. Heart Circ. Physiol. 286, 1223-1228 (2004).</li><li>Bishop, J. J., Nance, P. R., Popel, A. S., Intaglietta, M., Johnson, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+erythrocyte+aggregate+size+and+flow+rate+in+skeletal+muscle+venules.">Relationship between erythrocyte aggregate size and flow rate in skeletal muscle venules.</a> Am. J. Physiol. Heart Circ. Physiol. 286, H113-120 (2004).</li><li>Baldwin, A. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+hemoglobins+produce+venular+interendothelial+gaps+and+albumin+leakage+in+the+rat+mesentery.">Modified hemoglobins produce venular interendothelial gaps and albumin leakage in the rat mesentery.</a> Am. J. Physiol. Heart Circ. Physiol. 277, H650-H659 (1999).</li><li>Cabrales, P., Zanini, G. M., Meays, D., Frangos, J. A., Carvalho, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Murine+cerebral+malaria+is+associated+with+a+vasospasm-like+microcirculatory+dysfunction,+and+survival+upon+rescue+treatment+is+markedly+increased+by+nimodipine.">Murine cerebral malaria is associated with a vasospasm-like microcirculatory dysfunction, and survival upon rescue treatment is markedly increased by nimodipine.</a> Am. J. Pathol. 176 (3), 1306-1315 (2010).</li></ol>

ここで説明する生体顕微鏡法は、マウスの軟膜微小循環の詳細な観察のためのユニークで強力なツールが用意されています。それは個々の細動脈と細静脈を疎かし、そのような血管径、赤血球の速度、血流、白血球、血小板などの血液の要素の遵守とローリング、血管漏出、組織のpHとPO2のようなパラメータの数の変化を測定し、潜在的に多くのことが可能となりますアプリケーション。 ...

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intravital microscopy of the mouse brain microcirculation using a closed cranial window

この実験モデルは 、in vivo蛍光顕微鏡で使用して、急性および慢性の、生理学的および病態生理学的血行動態、炎症、代謝状態のときにマウスの軟膜微小循環を評価するために設計されました。閉じた頭蓋ウィンドウは、マウスの左頭頂後頭葉皮質の上に配置されています。地元の微小循環は、エピと蛍光の照明を使用してウィンドウを使ってリアルタイムに記録されており、血管径と赤血球（RBC）速度の測定が実行されます。 RBCの速度は、リアルタイムの相互相関および/または蛍光標識赤血球を用いて測定されます。軟膜血管と血流と血管の漏出の評価に白血球や血小板の付着は、アルブミン- FITCおよび抗CD45 - TXR抗体などの蛍光標識マーカーの助けを借りて作られています。微小循環は、数日間にわたって繰り返して、ビデオを記録することができます。我々は、血液中の深遠な減少をマウスのマラリアberghei ANKAの感染症の経過中に動的な変化に追従する軟膜微小循環を研究し、CMの発現は血管収縮を特徴と微小循環機能不全に関連付けられていることを示すために最初にウィンドウを閉じるの脳生体顕微鏡を用い流れと最終的には血管虚脱。

Watch this Scientific Journal Video about Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window at JoVE.com

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window

軟膜微小循環の時間的空間的血行動態および炎症性イベントに追従するように生体顕微鏡。

クローズ頭蓋窓を使用してマウス脳の微小循環の生体顕微鏡

This experimental model was designed to assess the mouse pial microcirculation during acute and chronic, physiological and pathophysiological hemodynamic, ...

Research

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免疫電子顕微鏡のためのマウスの脳組織の準備

Transmission electron microscopy (TEM) is extremely useful for visualizing microglial, oligodendrocytic, astrocytic, and neuronal subcellular compartments ...

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

マウス脳の長期イメージングのためのポリッシュと強化間引き頭蓋骨ウィンドウ

In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form ...

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YFPを発現クリアマウス脳の多光子顕微鏡

Multiphoton microscopy of intrinsic fluorescence and second harmonic generation (SHG) of whole mouse organs is made possible by optically clearing the ...

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear

再固定ウィンドウの間に絶滅の訓練は恐怖の回復を防ぎ

Fear is maladaptive when it persists long after circumstances have become safe. It is therefore crucial to develop an approach that persistently prevents ...

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

小脳顆粒ニューロンの遺伝子操作<em&gt;体外</em&gt;と<em&gt;インビボ</em&gt;ニューロンの形態と移行を研究するために、

Developmental events in the brain including neuronal morphogenesis and migration are highly orchestrated processes. In vitro and in vivo analyses allow ...

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

モデルとしての嗅覚系は、軸索の成長パターンと形態を研究する<em&gt;インビボ</em

The olfactory system has the unusual capacity to generate new neurons throughout the lifetime of an organism. Olfactory stem cells in the basal portion of ...

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

で閉鎖性頭部外傷性脳損傷を負わせる方法<em&gt;ショウジョウバエ</em

Traumatic brain injury (TBI) affects millions of people each year, causing impairment of physical, cognitive, and behavioral functions and death. Studies ...

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ピエゾ高精度手術小片除去 (フェーザ): マウスの改良された頭蓋ウィンドウ術のテクニック

Multiphoton microscopy has been widely adapted for imaging neurons in vivo. Repeated imaging requires implantation of a cranial window or repeated ...

Laminectomy and Spinal Cord Window Implantation in the Mouse

マウスにおける線込みおよび脊髄窓移植

This protocol describes a method for spinal cord laminectomy and glass window implantation for in vivo imaging of the mouse spinal cord. An integrated ...

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

蛍光抗体法を用いたマウス脳内シナプス蛋白質の不均質分布の定量化

The presence, absence, or levels of specific synaptic proteins can severely influence synaptic transmission. In addition to elucidating the function of a ...