Name: co-culture models of pseudomonas aeruginosa biofilms grown on live human airway cells
Uploaded: 2010-10-06T17:00:00
Description: Watch this Scientific Journal Video about Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells at JoVE.com

우리는이 모델을 개발 지침과 제안을 G. O 툴 감사하고 싶습니다. 이 작품은 낭성 섬유증 재단 (GGA, STANTO07RO 및 BAS에 STANTO08GA에 ANDERS06F0), 국립 보건원 (GGA 및 BAS에 R01 - HL074175에 T32A107363)에서 지원하고,되었다 바이오 메디컬 연구 우수 연구 자원 센터를위한 국립 센터 (꼬브르 BAS에 P20 - RR018787).

1. 정적 공동 문화 Biofilm 모델 <ol><li> 정적 공동 문화 Biofilm 모델 1 원래 ΔF508 - CFTR 돌연변이 2,3,4에 대한 CF의 homozygous와 개인의 발전 세포를 영원히 아르 CFBE41o - 세포 (CFBE 세포)를 사용합니다. CFBE 세포가 / 잘 6 잘 조직 배양 플레이트 또는 10% 태아 소 혈청과 보충 최소한의 필수적인 매체 24 잘 조직 배양 플레이트 (멤) 2 X 10 5 10 6 세포의 농도에 씨앗을해야합니다 2mM...

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Biofilms 환경 자극에 대한 응답으로 양식 박테리아의 커뮤니티입니다. 이러한 환경 신호 표면, 집합, exopolysaccharides 생산, 그리고 증가 항생 저항 10 다른 phenotypes에 바인딩에서 발생하는 각 세균 내에 세계적인 규제 변화로 이어집니다. 수십년 지난 몇 동안 많은 증거가 biofilms는 만성 감염의 pathogenesis에 큰 역할을한다는 가설을 지원하고 있습니다. 예를 들어, 그것은 잘 접수되었는지 P. ?...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>FCS2 (Focht Live-Cell) chamber</td>
<td>&nbsp;</td>
<td>Bioptechs, Butler, PA</td>
<td>060319131616</td>
<td>&nbsp;</td>
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<td>FCS2 chamber controller</td>
<td>&nbsp;</td>
<td>Bioptechs, Butler, PA</td>
<td>060319-2-0303</td>
<td>&nbsp;</td>
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<td>40 mm glass coverslips</td>
<td>&nbsp;</td>
<td>Bioptechs, Butler, PA</td>
<td>PH 40-1313-0319</td>
<td>&nbsp;</td>
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<td>MEM</td>
<td>&nbsp;</td>
<td>Mediatech, Manassass, VA</td>
<td>#10-010-CV</td>
<td>&nbsp;</td>
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<td>MEM without phenol red</td>
<td>&nbsp;</td>
<td>Mediatech, Manassass, VA</td>
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co-culture models of pseudomonas aeruginosa biofilms grown on live human airway cells

세균성 biofilms는 다양한 인간 질병의 숫자와 관련되어 있지만, biofilm 개발은 일반적으로 비 - 생활 표면에 연구되었습니다. 본 논문에서는, 우리는 문화의 성장 인간기도 상피 세포 (CFBE 세포)에 형성 녹농균의 biofilms에 대한 프로토콜을 설명합니다. 첫 번째 방법에서는 (정적 공동 문화 Biofilm 모델을 칭했다), P. 녹농균이라는 박테리아가 표준 조직 문화 접시에 합류 monolayers로 성장 CFBE 전지 incubated입니다. 박테리아가 상피 세포에 매우 독성이지만, 아르기닌 지연 monolayer의 파괴 또한 충분히 biofilms에 대한 CFBE 세포에서 만들어집니다. 두 번째 방법은 (흐름 전지 공동 문화 Biofilm 모델을 칭했다), 종종 CFBE 세포의 합류 monolayer를 지원하는 유리 coverslip을 수용하기 위해 biofilm 연구에 사용되는 biofilm 흐름 전지 장치의 적응을 포함한다. 이 monolayer는 P.과 주사입니다 녹농균이라는 박테리아와 연동 펌프 다음 세포에 걸쳐 신선한 매체를 흐르고 있습니다. 두 시스템에서 세균성 biofilms는 접종 후 6-8시간 이내에 양식. biofilm의 시각화는 P.의 사용에 의해 향상됩니다 녹농균이라는 박테리아의 변종은 녹색 형광 단백질 constitutively (GFP)을 표현. 정적 및 흐름 세포 공동 문화 Biofilm의 assays 일찍 P.을위한 모델 시스템 아르 낭성 섬유증 (CF) 폐, 이러한 기술의 녹농균이라는 박테리아 감염은 P.의 다양한 측면을 허용 녹농균이라는 박테리아의 biofilm 형성과 독성이 biofilm의 세포 독성, biofilm CFU 측정 및 biofilm을 얼룩과 시각화를 포함하여 공부 할 수 있습니다.

Watch this Scientific Journal Video about Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells at JoVE.com

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

본 논문은 성장의 다른 방법을 설명합니다<em&gt; 녹농균</em&gt; 교양 인간기도 상피 세포에서 biofilms. 이러한 프로토콜은 biofilm 단위 (CFU)를 형성 식민지를 측정, biofilm의 얼룩 및 biofilm의 세포 독성을 공부, biofilm의 시각화를 포함한 biofilm 형성의 다양한 측면을 연구 적용할 수 있습니다.

공동 문화 모델 녹농균 Biofilms는 라이브 인간 에어웨이 세포에 들어라

Bacterial biofilms have been associated with a number of different human diseases, but biofilm development has generally been studied on non-living ...

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Immunology and Infection

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

흐름 세포 녹농균 및 Saccharomyces cerevisiae의의 Biofilm

Many microbial cells have the ability to form sessile microbial communities defined as biofilms that have altered physiological and pathological ...

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HIV-1 감염 CD4의 타고난 감지 평가<sup&gt; +</sup&gt;를 사용하여 형질 수지상 세포에 의한 T 세포<em&gt; 예 생체 내</em&gt; 공동 문화 시스템.

HIV-1 innate sensing requires direct contact of infected CD4+ T cells with plasmacytoid dendritic cells (pDCs). In order to study this process, the ...

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주문, 녹 농 균 스트레인 PA14 Transposon 삽입 돌연변이의 비중복 라이브러리의 복제

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Patients who survive pneumonia have elevated death rates in the months following hospital discharge. It has been hypothesized that infection of pulmonary ...

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Pseudomonas aeruginosa, an opportunistic Gram-negative bacterial pathogen, can overproduce an exopolysaccharide alginate resulting in a unique phenotype ...

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