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DOI :
10.3791/2195-v
December 18th, 2010
Chapters
0:05
Title
1:04
Introduction
1:17
Thawing and Pre-Activation of CD34+ Cells
3:25
Retroviral Transduction by Virus Pre-Loading
5:04
Cell Sorting and Collection of Transduced Cells
6:08
CFC Assay
8:14
Giemsa Staining
10:29
CFC Assay Results with Geimsa Stain Analyses
11:07
Conclusion
集落形成细胞(CFC)的检测是在体外实验中,造血祖细胞形成半固体培养基中的菌落。结合菌落形态,细胞形态,流式细胞仪是用来评估的祖细胞的增殖和分化沿不同的造血谱系能力。
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