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DOI :
10.3791/2195-v
December 18th, 2010
Chapters
0:05
Title
1:04
Introduction
1:17
Thawing and Pre-Activation of CD34+ Cells
3:25
Retroviral Transduction by Virus Pre-Loading
5:04
Cell Sorting and Collection of Transduced Cells
6:08
CFC Assay
8:14
Giemsa Staining
10:29
CFC Assay Results with Geimsa Stain Analyses
11:07
Conclusion
細胞(CFC)アッセイをコロニー形成は、造血前駆細胞が半固形培地でコロニーを形成しているのin vitroアッセイである。コロニー形態、細胞形態、およびフローサイトメトリーの組み合わせは、異なる造血系統に沿って増殖し、分化する前駆細胞の能力を評価するために使用されます。
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