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DOI :
10.3791/2198-v
•
9:53 min
September 23rd, 2010
Chapters
0:00
Title
1:15
Plasmid Transfection to Produce Rev-dependent Lentiviral Particles
4:24
Concentrating Viral Particles and Determining Viral Titer
6:15
Marking HIV-1 Positive Cells
8:25
Identifying HIV-positive Cells
9:30
Conclusion
我々はTAT -応答LTR、HIV - 1 TAT -とRev依存的にレポーター遺伝子の発現を調節することができるREV -応答エレメント(RRE)に加えて、所有しているレンチウイルスベクターを開発した。ベクトルは、GFPの発現を介して、生細胞における複製HIVの特異的検出を可能にする。
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