Name: technique to collect fungiform (taste) papillae from human tongue
Uploaded: 2010-09-18T17:00:00
Description: Watch this Scientific Journal Video about Technique to Collect Fungiform Taste Papillae from Human Tongue at JoVE.com

这项工作是支持由美国国立卫生研究院R21 DC03969 - 01（日本国）。我们感谢弗里茨Lischka博士捕获RT - PCR检测显示在图4B和先生，贝利和S.女士阿拉尔孔技术援助执行单一的味蕾细胞，博士Tauf Huque。 M.亚尼纳Pepino是一个NIDAT32 DA07313华盛顿大学医学院，圣路易斯，MO（Cottler，LB PI）支持的一个老乡。

1。介绍和一般注意事项一般程序包括一个小的（1厘米2）面积的前舌背表面，去除一个（6-8）（味）的菌状乳头的数量，麻醉面积使用一个弯曲的弹簧微局部麻醉 - 剪刀（麦弗逊式Vannas类型＃简5603，Roboz，罗克维尔，医学博士）。删除的菌状乳头约0.25 毫米的 3个。有很少的非乳头（味道）去除上皮组织的每个乳头。新乳头的形式，在这一领域经过3至5个星期，...

<ol>
	<li>Breslin, P. A., Spector, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mammalian+taste+perception.">Mammalian taste perception.</a> Curr Biol. 26 (4), R148-R155 (2008).</li><li>Miller, I., Doty, R. I., Dekker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anatomy+of+the+peripheral+taste+system.">Anatomy of the peripheral taste system.</a> Handbook of Olfaction and Gustation. , 521-547 (1995).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Figure+12.67.">Figure 12.67.</a> Gray's Anatomy: The Anatomical Basis of Medicine and Surgery. , (1995).</li><li>Huque, T., Cowart, B. J., Dankulich-Nagrudny, L., Pribitkin, E. A., Bayley, D. L., Spielman, A., Feldman, I., S, R., Mackler, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sour+ageusia+in+two+individuals+implicates+ion+channels+of+the+ASIC+and+PKD+families+in+human+sour+taste+perception+at+the+anterior+tongue.">Sour ageusia in two individuals implicates ion channels of the ASIC and PKD families in human sour taste perception at the anterior tongue.</a> PLoS One. 4 (10), e7347-e7347 (2009).</li><li>MacDonald, J. F., Mody, I., Salter, M. W., Spielman, A. I., Spielman, A. I., Brand, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patch-clamping+of+taste+cells+in+mouse.">Patch-clamping of taste cells in mouse.</a> Experimental Cell Biology of Taste and Olfaction. , 329-332 (1995).</li></ol>

使用人体细胞的一个显着的优势是其特殊性。在这里详细介绍的过程提供了来自人类的味觉受体细胞的分离舌组织。这些味觉细胞，可用于分子生物学研究，试图发现味觉信息传导的重要分子，监测活动，以刺激味觉细胞的钙成像研究。此外，此过程中提供的舌组织可与各种技术， 如原位杂交，免疫组织化学分析，并允许捕捉如聚合酶链反应（PCR）技术，包括实时定量单细胞PCR的分...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>McPherson-Vannas. Curved, sharp cutting edge 5mm. Comb, tip width 0.2 mm overall length 3"</td>
<td>&nbsp;</td>
<td>Roboz Surgical Instruments Co., Inc. Gaithersburg, MD 20898</td>
<td>RS-5603, Roboz</td>
<td>&nbsp;</td>
<tr>
<td>Small Dumont forceps</td>
<td>&nbsp;</td>
<td>Roboz Surgical Instruments Co.</td>
<td>RS-4915, Roboz</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

technique to collect fungiform (taste) papillae from human tongue

味觉是人类生活的关键。通知将潜在摄入的食物质量的身体和刺激，准备消化道的消化代谢过程。对了解早期生物化学和分子事件的基本味道传导（检讨，布雷斯林和斯佩克特，2008年1）正在取得稳步进展。然而，迄今取得的进展在很大程度上导致动物模型。然而，由于在受体特异性和受体密度显着差异有所不同物种之间，人类的味道传导只会被理解，使用人类的味道组织。在这里，我们描述了一个活检技术，人类菌状乳头，可见圆形粉红色的前结构，收集包含味蕾直径约0.5毫米。这些切片乳头被用于多种用途，包括可行的味蕾细胞的分离 ，原位杂交，免疫组织化学，并通过分子生物学技术的，识别特定味道的新型蛋白质。

Watch this Scientific Journal Video about Technique to Collect Fungiform Taste Papillae from Human Tongue at JoVE.com

Technique to Collect Fungiform Taste Papillae from Human Tongue

基本味觉转导的分子机制方面的知识最近享受到了重大进展，主要是由于使用动物模型。然而，在味觉的灵敏度和特异性哺乳动物认股权证在人体组织的研究广泛​​的多样性。我们描述了一个活检技术，收集从对人舌乳头的生活品位细胞。

技术收集人舌菌状（味道）乳头

The sense of taste is critical for human life. It informs the body about the quality of food that will be potentially ingested and stimulates metabolic ...

Research

JoVE Journal

Medicine

Technique to Collect Fungiform (Taste) Papillae from Human Tongue

Isolation of Human Islets from Partially Pancreatectomized Patients

从部分Pancreatectomized患者的人类胰岛的分离

Investigations into the pathogenesis of type 2 diabetes and islets of Langerhans malfunction 1 have been hampered by the limited availability of type 2 ...

科研

医学

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe

简化技术<em&gt;原位</em&gt;切除角膜和视网膜组织剜人眼地球

Enucleation is the process of retrieving the ocular globe from a cadaveric donor leaving the rest of the globe undisturbed. Excision refers to the ...

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&#246;m Technique

Human Skeletal Muscle Biopsy Procedures Using the Modified BergstrÃƒÆ’Ã‚Â¶m Technique

人骨骼肌活检手术使用修改伯格斯坦技术

The percutaneous biopsy technique enables researchers and clinicians to collect skeletal muscle tissue samples. The technique is safe and highly ...

Using a Laminating Technique to Perform Confocal Microscopy of the Human Sclera

使用层压技术来执行人类巩膜共聚焦显微镜


The sclera is a dense connective tissue that covers and protects the eye. It mainly consists of dense collagen bundles (types I, III, IV, V, VI, and ...

In Vivo Evaluation of the Mechanical and Viscoelastic Properties of the Rat Tongue

In Vivo Evaluation of the Mechanical and Viscoelastic Properties of the Rat Tongue

大鼠舌的机械和粘弹性的体内评估

The tongue is a highly innervated and vascularized muscle hydrostat on the floor of the mouth of most vertebrates. Its primary functions include ...

Technique of Minimally Invasive Transverse Aortic Constriction in Mice for Induction of Left Ventricular Hypertrophy

小鼠横主动脉狭窄诱导左心室肥厚的技术研究

Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure ...

Taste Exam: A Brief and Validated Test

味觉考试: 一个简短的验证测试

The emerging importance of taste in medicine and biomedical research, and new knowledge about its genetic underpinnings, has motivated us to supplement ...

Standardized Technique of Aortic Valve Re-implantation for Valve-sparing Aortic Root Replacement

主动脉瓣重置术治疗瓣膜备用主动脉根置换的规范化技术

Despite the obvious advantages of the preservation of a normal aortic valve during aortic root replacement, the complexity of valve sparing procedures ...

Characterization of a Novel Human Organotypic Retinal Culture Technique

新型人类组织特征视网膜培养技术的特征

Previous human organotypic retinal culture (HORC) models have utilized detached retinas; however, without the structural support conferred by retinal ...

Psychophysical Tracking Method to Assess Taste Detection Thresholds in Children, Adolescents, and Adults: The Taste Detection Threshold (TDT) Test

Psychophysical Tracking Method to Assess Taste Detection Thresholds in Children, Adolescents, and Adults: The Taste Detection Threshold TDT Test

评估儿童、青少年和成人味觉检测阈值的心理物理跟踪方法：味觉检测阈值（TDT）测试

This paper describes a two-alternative, forced-choice, staircase, tracking procedure, called the Taste Detection Threshold (TDT) test, that provides a ...