JoVE Logo
Faculty Resource Center

Sign In

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

DOI :

10.3791/2203-v

August 7th, 2010

August 7th, 2010

12,495 Views

1Epigenetics in Human Health and Disease, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenomic Medicine, BakerIDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, University of Melbourne

Microscopic analysis of γH2AX foci, which form following the phosphorylation of H2AX at Ser-139 in response to DNA double-strand breaks, has become an invaluable tool in radiation biology. Here we used an antibody to mono-methylated histone H3 at lysine 4 as an epigenetic marker of actively transcribing euchromatin, to evaluate the spatial distribution of radiation-induced γH2AX formation within the nucleus.

Tags

Spatial Distribution

-- Views

Related Videos

article

Quantification of γH2AX Foci in Response to Ionising Radiation

article

The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

article

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

article

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

article

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System

article

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

article

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules

article

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

article

Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

article

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved