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12.5K Views
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09:28 min
August 7th, 2010
DOI :
10.3791/2203-v
Chapters
0:00
Title
0:05
Introduction
1:01
Cell Preparation
2:28
Immunofluorescence Staining
8:39
Analysis 3D-Image Reconstruction
9:09
Conclusion
5:00
Microscopy
6:12
Analysis Foci Counting
7:56
Analysis Line Scan
Summary
微观分析γH2AX灶,形成以下丝氨酸139磷酸化H2AX在DNA双链断裂,已成为辐射生物学中的一个非常宝贵的工具。在这里,我们使用一种抗体,单甲基化的组蛋白H3赖氨酸4作为一个积极抄写常染色质的表观遗传标记,以评估辐射诱导γH2AX形成的细胞核内的空间分布。
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