Name: a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment
Uploaded: 2010-08-27T17:30:00
Description: Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

हमारा काम NIH / NIGMS, NSF, USDA NIFA /, और चारण से कुलपति को अनुदान द्वारा समर्थित है.

पृष्ठभूमि संयंत्र कहनेवाला कनेक्शन के माध्यम से बड़े अणुओं के Symplastic परिवहन, plasmodesmata, कई संयंत्र pathologists और जीव के लिए ब्याज की है. उदाहरण के लिए, कई वायरल प्रोटीन plasmodesmal आकार अपवर्जन सीमा को विनियमित क?...

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	<li>Waigmann, E., Ueki, S., Trutnyeva, K., Citovsky, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+ins+and+outs+of+non-destructive+cell-to-cell+and+systemic+movement+of+plant+viruses.+.">The ins and outs of non-destructive cell-to-cell and systemic movement of plant viruses. .</a> Crit Rev Plant Sci. 23, 195-250 (2004).</li><li>Lucas, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Plant+viral+movement+proteins:+agents+for+cell-to-cell+trafficking+of+viral+genomes.">Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes.</a> Virology. 344, 169-184 (2006).</li><li>Epel, B. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Plant+viruses+spread+by+diffusion+on+ER-associated+movement-protein-rafts+through+plasmodesmata+gated+by+viral+induced+host+beta-1,3-glucanases.">Plant viruses spread by diffusion on ER-associated movement-protein-rafts through plasmodesmata gated by viral induced host beta-1,3-glucanases.</a> Semin Cell Dev Biol. 20, 1074-1081 (2009).</li><li>Zambryski, P. C., Crawford, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Plasmodesmata:+gatekeepers+for+cell-to-cell+transport+of+developmental+signals+in+plants.">Plasmodesmata: gatekeepers for cell-to-cell transport of developmental signals in plants.</a> Annu Rev Cell Dev Biol. 16, 393-421 (2000).</li><li>Rivero-Lepinckas, L., Crist, D., Scholl, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+of+plants+and+presercation+of+seeds.">Growth of plants and presercation of seeds.</a> Methods Mol Biol. 323, 3-12 (2006).</li><li>Ueki, S., Lacroix, B., Krichevsky, A., Lazarowitz, S. G., Citovsky, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+transient+genetic+transformation+of+Arabidopsis+leaves+by+biolistic+bombardment.">Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment.</a> Nat Protoc. 4, 71-77 (2009).</li><li>Oparka, K. 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symplastic परिवहन परख की सफलता के लिए कुंजी को उच्च परिवर्तन दक्षता है, जो सांख्यिकीय महत्वपूर्ण है और आसानी से detectible संकेत समूहों के उत्पादन की अनुमति देता प्राप्त है. यह स्वस्थ, मजबूत पौधों से काटा पत्तियों क...

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<td>Gold microparticles, 1.0 &mu;m in diameter</td>
<td>&nbsp;</td>
<td>Bio-Rad</td>
<td>165-2262</td>
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<td>Bio-Rad</td>
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<td>S0266-1G</td>
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<td>&nbsp;</td>
<td>Bio-Rad</td>
<td>165-2441</td>
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a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment

यहाँ, हम एक सरल और तेजी से सेल सेल Planta में macromolecular परिवहन की सीमा का पता लगाने और आकलन के लिए प्रोटोकॉल उपस्थित थे. इस प्रोटोकॉल में, एक fluorescently ब्याज की टैग प्रोटीन transiently अपने एन्कोडिंग डीएनए के biolistic निर्माण प्रसव के बाद संयंत्र के ऊतकों में व्यक्त किया. टैग प्रोटीन का अंतर और कहनेवाला वितरण तो confocal माइक्रोस्कोपी द्वारा विश्लेषण किया है. हम विस्तार में इस प्रौद्योगिकी का वर्णन है, परख के लिए कदम दर कदम प्रोटोकॉल प्रदान करने और तीन प्रजातियों के पौधे में symplastic प्रोटीन परिवहन की हद तक, Arabidopsis thaliana, Nicotiana benthamiana और एन का मूल्यांकन tabacum (तम्बाकू).

Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

संयंत्र कोशिकाओं के बीच macromolecular तस्करी transiently ब्याज की एक प्रोटीन fluorescently टैग व्यक्त और confocal माइक्रोस्कोपी द्वारा अपने अंतर और कहनेवाला वितरण का विश्लेषण करके मूल्यांकन किया जा सकता है.

एक सेल सेल macromolecular परिवहन परख Planta</emउपयोग> Biolistic बमबारी

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a ...

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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

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