Name: a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment
Uploaded: 2010-08-27T17:30:00
Description: Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

Nuestro trabajo es apoyado por becas de NIH / NIGMS, NSF, USDA / Nifa, y BARD a VC.

Fondo Simplástica transporte de macromoléculas a través de conexiones intercelulares de plantas, los plasmodesmos, es de interés para muchos patólogos de plantas y biólogos. Por ejemplo, varias proteínas virales son conocidas para regular plasmodesmal límites de exclusión de tamaño para permitir el movimiento viral 1-3. Además, algunas proteínas endógenas, entre ellos importantes reguladores de desarrollo, se supone que se mueven de célula a célula, presumiblemente a ...

<ol>
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La clave para el éxito del ensayo de transporte simplástica es la obtención de la eficiencia de transformación de alto, que permite la producción de racimos de señal estadísticamente significativa y detectable fácilmente. Esto puede lograrse utilizando las hojas cosechadas de plantas sanas, robustas, y la preparación de partículas de oro recubiertas por una preparación de ADN puro y concentrado. Utilizando las hojas en la misma etapa de crecimiento también es vital para la fiabil...

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a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment

A continuación, presentamos un protocolo sencillo y rápido para detectar y evaluar la magnitud de la célula a célula de transporte macromolecular en la planta. En este protocolo, marcado con fluorescencia de proteínas de interés se expresa de forma transitoria en el tejido vegetal después de la entrega biolística de su ADN que codifica para la construcción. La distribución intra e intercelulares de la proteína marcada se analiza por microscopía confocal. Se describe en detalle esta tecnología, ofreciendo paso a paso a protocolos de ensayo y evaluar la magnitud del transporte de proteínas simplástica en tres especies de plantas, Arabidopsis thaliana, Nicotiana benthamiana y N. tabacum (tabaco).

Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

El tráfico de macromoléculas entre las células vegetales se pueden evaluar de forma transitoria que expresa una proteína fluorescente con etiquetas de interés y el análisis de su distribución intra e intercelulares mediante microscopia confocal.

Una célula a célula macromoleculares de transporte de ensayo En la Planta La utilización de bombardeo biolística

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a ...

Research

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Biology

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

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The intestinal epithelium has important transport and barrier functions that play key roles in normal physiological functions of the body while providing ...

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