Name: a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment
Uploaded: 2010-08-27T17:30:00
Description: Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

Bizim işimiz, VC NIH / NIGMS, NSF, USDA / NIFA ve BARD hibe tarafından desteklenmektedir.

Arkaplan Bitki hücrelerarası bağlantıları aracılığıyla makromoleküllerin Symplastic ulaşım, plasmodesmata, birçok bitki patologlar ve biyologlar ilgi. Örneğin, bazı viral proteinler, viral hareketi 1-3 etkinleştirmek için plasmodesmal boyut dışlama sınırlarını düzenlemek için bilinmektedir . Ayrıca, önemli gelişim düzenleyiciler arasında bazı endojen proteinler, 4-hücreli olmayan-özerk çalışması için, muhtemelen plasmodesmata aracıl...

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Symplastic taşıma testinin başarı için anahtar, yüksek dönüşüm verimliliği, istatistiksel olarak anlamlı ve kolayca detectible sinyal kümeleri üretimini sağlar elde etmektir. Bu, sağlıklı, sağlam bitkiler hasat yapraklar kullanılarak, saf ve yoğun bir DNA hazırlığı ile kaplı altın parçacıkları hazırlanması sağlanabilir. Aynı büyüme aşamasında yaprakları kullanarak da testin güvenilirliği için hayati önem taşımaktadır. Plasmodesmal diyafram farkl?...

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<td>Gold microparticles, 1.0 &mu;m in diameter</td>
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a cell-to-cell macromolecular transport assay in planta utilizing biolistic bombardment

Burada, planta içinde hücre-hücre makromoleküler taşıma kapsamını tespit etmek ve değerlendirmek için basit ve hızlı bir protokol mevcut. Bu protokol, geçici bir ilgi floresan etiketli protein kodlama DNA Biyolistik teslim inşa aşağıdaki bitki doku ifade edilir. Etiketli protein içi ve hücreler arası dağılımı sonra konfokal mikroskobu ile analiz edilir. Biz detaylı olarak tahlil için adım adım protokoller sağlayarak, bu teknoloji tanımlayan ve üç bitki türlerinin symplastic protein taşıma ölçüde, Arabidopsis thaliana, Nicotiana benthamiana ve N. değerlendirmek tabacum (tütün).

Watch this Scientific Journal Video about A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment at JoVE.com

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Bitki hücreleri arasındaki makromoleküler ticareti, geçici bir ilgi floresan etiketli protein ifade ve konfokal mikroskobu ile içi ve hücreler arası dağılımı analiz değerlendirilebilir.

-Cell Hücre makromoleküler Ulaştırma Testi Planta Biyolistik Bombardıman kullanımı

Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a ...

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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

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In this protocol, we present the required materials, and the procedure for making modified&#160;C. elegans&#160;Habituation and Reproduction media ...

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The intestinal epithelium has important transport and barrier functions that play key roles in normal physiological functions of the body while providing ...

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Live imaging of the pHluorin tagged Soluble N-ethylmaleimide-sensitive-factor Attachment protein REceptor (v-SNARE) Vesicle-associated membrane protein 7 ...

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The use of neutron macromolecular crystallography (NMX) is expanding rapidly with most structures determined in the last decade thanks to new NMX ...