Name: maintaining wolbachia in cell-free medium
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

maintaining wolbachia in cell-free medium

在这个视频协议，证明程序（1）净化的Wolbachia共生菌的培养蚊子细胞，（2）使用荧光检测，以确定纯化的Wolbachia的可行性及（3）保持在无细胞培养基外的Wolbachia 。纯净的Wolbachia仍然活着，但在细胞阶段不进行复制，直到进入真核细胞重新接种。外的Wolbachia纯化以这种方式将保持在室温下至少一个星期可行，可能更长。纯净的Wolbachia适合显微注射，DNA提取和其他应用。

Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

Maintaining Wolbachia in Cell-free Medium

本视频介绍了净化按蚊细胞系的Wolbachia pipientis，然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。

维持细胞培养基的Wolbachia

In this video protocol, procedures are demonstrated to (1) purify Wolbachia symbionts out of cultured mosquito cells, (2) use a fluorescent assay to ...

Research

JoVE Journal

Biology

Wolbachia Bacterial Infection in Drosophila

在果蝇的Wolbachia细菌感染

科研

生物学

Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive

人口控制媒介种群和遗传的驱动器使用的Wolbachia pipientis替换策略

In this video, Jason Rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "Population ...

Assaying Proteasomal Degradation in a Cell-free System in Plants

测定蛋白酶体降解的植物无细胞系统

The ubiquitin-proteasome pathway for protein degradation has emerged as one of the most important mechanisms for regulation of a wide spectrum of cellular ...

Bone Conditioned Medium: Preparation and Bioassay

骨条件培养基的制备和生物测定

Autologous bone grafts are widely used in oral and maxillofacial surgery, orthopedics, and traumatology. Autologous bone grafts not only replace missing ...

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

基于海拉细胞自由表达系统中的表达<em&gt;疟原虫</em&gt;棒状体蛋白

Malaria causes significant global morbidity and mortality. No routine vaccine is currently available. One of the major reasons for lack of a vaccine is ...

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

无细胞化验，研究染色质解聚在有丝分裂的结束

During the vertebrate cell cycle chromatin undergoes extensive structural and functional changes. Upon mitotic entry, it massively condenses into rod ...

Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

在大鼠提睾肌的小动脉的可视化和量化的无细胞层

The cell-free layer is defined as the parietal plasma layer in the microvessel flow, which is devoid of red blood cells. The measurement of the in vivo ...

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

维护伊蚊蚊蚊子感染沃尔巴克

Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, ...

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

中通量筛选法评估对精子中 ca2 +信号和反应的影响

Ca2+-signaling is essential to normal sperm cell function and male fertility. Similarly, the acrosome reaction is vital for the ability of a human sperm ...

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

快速、经济、简单地生产无细菌细胞裂解物

Cell-free gene expression offers the power of biology without the complications of a living organism. Although many such gene expression systems exist, ...