Name: maintaining wolbachia in cell-free medium
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

maintaining wolbachia in cell-free medium

Dans ce protocole vidéo, des procédures sont mises en évidence (1) d&#39;épurer les symbiotes Wolbachia hors des cellules de moustique cultivé, (2) utiliser un test de fluorescence pour déterminer la viabilité de la bactérie Wolbachia purifiée et (3) maintenir les Wolbachia désormais extracellulaire dans la cellule du milieu sans . Purifié par Wolbachia rester en vie dans la phase extracellulaire mais ne répliquent pas jusqu&#39;au re-inoculée dans les cellules eucaryotes. Wolbachia extracellulaires purifiés de cette manière restera viable pour au moins une semaine à température ambiante, et peut-être plus. Purifié par Wolbachia sont adaptés à la micro-injection, extraction d&#39;ADN et d&#39;autres applications.

Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

Maintaining Wolbachia in Cell-free Medium

Cette vidéo décrit un procédé de purification Wolbachia pipientis partir d&#39;une ligne de cellules Anopheles gambiae et la culture de la endosymbiote dans la cellule sans moyen. Un test pour la viabilité de la bactérie est démontrée.

Le maintien de Wolbachia dans la cellule sans moyen

In this video protocol, procedures are demonstrated to (1) purify Wolbachia symbionts out of cultured mosquito cells, (2) use a fluorescent assay to ...

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Biology

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In this video, Jason Rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "Population ...

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