Name: maintaining wolbachia in cell-free medium
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

maintaining wolbachia in cell-free medium

In questo protocollo video, le procedure sono dimostrati a (1) purificare simbionti Wolbachia fuori delle cellule in coltura zanzare, (2) usare un test di fluorescenza per verificare la fattibilità del Wolbachia purificata e (3) mantenere la Wolbachia ora extracellulare in cell-free medio . Purificata Wolbachia rimanere vivo nella fase extracellulare, ma non replicare fino a quando re-inoculati in cellule eucariotiche. Wolbachia extracellulari purificati in questo modo rimarrà valida per almeno una settimana a temperatura ambiente, e forse più a lungo. Purificato Wolbachia sono adatti per la microiniezione, estrazione del DNA e altre applicazioni.

Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

Maintaining Wolbachia in Cell-free Medium

Questo video descrive un metodo per purificare Wolbachia pipientis da una linea di cellule Anopheles gambiae e poi la coltura endosymbiont nella cella senza medium. Un test per la vitalità del batterio è dimostrata.

Il mantenimento di Wolbachia in Cell-free medio

In this video protocol, procedures are demonstrated to (1) purify Wolbachia symbionts out of cultured mosquito cells, (2) use a fluorescent assay to ...

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Biology

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