Name: maintaining wolbachia in cell-free medium
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

maintaining wolbachia in cell-free medium

このビデオプロトコルで、手順は（1）、培養蚊細胞のボルバキアの共生を浄化（2）精製ボルバキアの生存を確認する蛍光アッセイを使用し、（3）細胞を含まない培地で今外ボルバキアを維持することが実証されています。精製ボルバキアは、細胞外相で生きているままですが、真核細胞に再接種するまで複製されません。この方法で精製された細胞外ボルバキアは、室温で少なくとも1週間実行可能なままであり、おそらく長くなります。精製ボルバキアは、マイクロインジェクション、DNAの抽出および他のアプリケーションに適しています。

Watch this Scientific Journal Video about Maintaining Wolbachia in Cell-free Medium at JoVE.com

Maintaining Wolbachia in Cell-free Medium

このビデオは、ハ​​マダラカの細胞株とし、無細胞培地で培養endosymbiontをからボルバキアpipientisを精製する方法を説明します。細菌の生存のためのアッセイは、示されている。

無細胞培地でボルバキアの維持

In this video protocol, procedures are demonstrated to (1) purify Wolbachia symbionts out of cultured mosquito cells, (2) use a fluorescent assay to ...

Research

JoVE Journal

Biology

Wolbachia Bacterial Infection in Drosophila

ショウジョウバエにおけるボルバキア細菌感染

Population Replacement Strategies for Controlling Vector Populations and the Use of Wolbachia pipientis for Genetic Drive

遺伝ドライブのための制御ベクトルの集団とボルバキアpipientisの使用のための人口置換戦略

In this video, Jason Rasgon discusses population replacement strategies to control vector-borne diseases such as malaria and dengue. "Population ...

Assaying Proteasomal Degradation in a Cell-free System in Plants

植物では無細胞系でプロテアソームによる分解をアッセイ

The ubiquitin-proteasome pathway for protein degradation has emerged as one of the most important mechanisms for regulation of a wide spectrum of cellular ...

Bone Conditioned Medium: Preparation and Bioassay

骨馴化培地：調製およびバイオアッセイ

Autologous bone grafts are widely used in oral and maxillofacial surgery, orthopedics, and traumatology. Autologous bone grafts not only replace missing ...

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins

の発現のためのHeLaベースの無細胞発現系<em&gt;マラリア原虫</em&gt; Rhoptryタンパク質

Malaria causes significant global morbidity and mortality. No routine vaccine is currently available. One of the major reasons for lack of a vaccine is ...

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

無細胞アッセイは、有糸分裂の終了時クロマチン脱凝縮を勉強します

During the vertebrate cell cycle chromatin undergoes extensive structural and functional changes. Upon mitotic entry, it massively condenses into rod ...

Visualization and Quantification of the Cell-free Layer in Arterioles of the Rat Cremaster Muscle

ラット精巣挙筋の細動脈における無細胞層の可視化と定量化

The cell-free layer is defined as the parietal plasma layer in the microvessel flow, which is devoid of red blood cells. The measurement of the in vivo ...

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

ネッタイシマカ蚊ボルバキアに感染維持

Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, ...

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

Ca2 +の効果の評価のためのスクリーニング アッセイ中スループット-シグナリングおよびひと精子先体反応

Ca2+-signaling is essential to normal sperm cell function and male fertility. Similarly, the acrosome reaction is vital for the ability of a human sperm ...

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

細菌無細胞リザートの迅速、手頃な価格、および単純な生産

Cell-free gene expression offers the power of biology without the complications of a living organism. Although many such gene expression systems exist, ...