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DOI :
10.3791/2236-v
•
8:29 min
July 15th, 2010
Chapters
0:04
Title
0:45
Introduction
1:33
Preparing Geltrex-coated Culture Dishes
2:59
Adapting Human iPSCs
5:20
Passaging Human iPSCs
7:36
Representative Results
8:07
Conclusion
以下のプロトコルは、完全なノックアウト血清代替のフィーダーフリー培地(KSR - FF)を使用して、フィーダーフリー文化に人間の人工多能性幹(iPS)細胞を適応させるための命令が用意されています。適応一度、継続的に維持するための手順も記載されています。
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