Name: induction and clinical scoring of chronic-relapsing experimental autoimmune encephalomyelitis
Uploaded: 2007-07-04T00:00:00
Description: Watch this Scientific Journal Video about Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis at JoVE.com

<p class="jove_title">慢性复发性EAE的感应和监测</p><p class="jove_content">乳液是一种抗原在水溶液中添加补充完整弗氏佐剂1:1混合。添加抗原的辅助，而不是反过来，这是非常重要的！ </p><p class="jove_content">决策和乳状液注射时，有大量的浪费。始终准备1.5或2倍，比你所需要的的更多。 </p><p class="jove_content"> <span class="jove_content">使用蒸压砂浆，杵，玻璃注射器，和桥梁。所有塑料应无菌。常规实验室长凳上，可以做的</spa...

<p>有一个本议定书的几个重要的考虑因素。 </p><p> DA大鼠的遗传学会略有不同，取决于饲养员他们都买了从。这些差异可能会增加或减少对大鼠EAE诱导的易感性。如果您的老鼠更容易受到EAE的，你会想，以减少使用非辅以完全弗氏佐剂甚至不完全弗氏佐剂免疫的强度。您也可以考虑降低乳液的量注射或用生理盐水稀释脊髓匀浆前准备的乳液。如果你的老鼠不发展EAE的临床体征，使用的全实力免疫乳液检查您的住房?...

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<td>Complete Freund's Adjuvant</td>
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<td>Mycobacterium tuberculosis H37Ra</td>
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<td>Fisher</td>
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<td>DA rat</td>
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<td>Harlan Sprague Dawley</td>
<td>&nbsp;</td>
<td>Females, 7-9 weeks old.</td>
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<td>Glass syringes - 5 ml</td>
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<td>Metal bridge - 18G</td>
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<td>Fisher</td>
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<td>Becton Dickinson</td>
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<td>Needles - 20G 1 1/2</td>
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induction and clinical scoring of chronic-relapsing experimental autoimmune encephalomyelitis

<p class="jove_content">多发性硬化症（MS），是一种慢性中枢神经系统（CNS），常见的影响青壮年炎症性疾病。它的特点是传播领域在大脑和脊髓脱髓鞘和胶质吓唬。这些病变改变神经传导和诱导禁用在中枢神经系统的脱髓鞘斑（如截瘫，瘫痪，失明，大小便失禁）的位置不同的神经功能缺损。 </p><p class="jove_content">实验性自身免疫性脑脊髓炎（EAE）是一个用于MS的模式。 EAE的是第一次不小心引起​​的在人类对狂犬病疫苗接种期间，利用对兔脊髓种植的病毒。脊髓注射病毒灭活残留引起的中枢神经系统疾病。根据这些意见，对EAE的第一种模式是在与中枢神经系统匀浆河流并于1935年Schwenther的免疫的非人类灵长类动物。脑脊髓炎以来一直产生多种物种，并可以按照不同的课程，取决于物种/应变和免疫抗原用于。例如，髓鞘碱性蛋白与佐剂乳化免疫Lewis大鼠诱导EAE的急性模型，而同一抗原诱导豚鼠慢性疾病。 </p><p class="jove_content">对DA大鼠脊髓完全弗氏佐剂乳化的免疫DA大鼠EAE模型，这里描述的是诱发。大鼠免疫接种后7-14天内开发一个递增的弛缓性麻痹。临床症状，遵循了几个星期的一个复发 - 缓解的过程中。病理显示在中枢神经系统脱髓鞘斑大的免疫浸润。特别考虑照顾与EAE动物在视频结束。 </p>

Watch this Scientific Journal Video about Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis at JoVE.com

Induction and Clinical Scoring of Chronic-Relapsing Experimental Autoimmune Encephalomyelitis

<p class="jove_content">本视频演示诱导多发性硬化症的动物模型和临床评分标准：慢性复发DA大鼠实验性自身免疫性脑脊髓炎。这种疾病，诱发免疫大鼠包含整个大鼠脊髓和完全弗氏佐剂乳化，呈现类似人类疾病的临床体征。</p>

归纳和慢性复发性实验性自身免疫性脑脊髓炎的临床评分

Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that commonly affects young adults. It is characterized by ...

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