Name: dissection of oenocytes from adult drosophila melanogaster
Uploaded: 2010-07-18T17:00:00
Description: Watch this Scientific Journal Video about Dissection of Oenocytes from Adult Drosophila melanogaster at JoVE.com

我们想感谢她的协助，在拍摄这段视频文章Amsale扎绳。这项工作是由CIHR，NSERC和CRC赠款JDL。

第1部分。圆角准备的成年果蝇腹部。 立即在下面的准备过程概述腹部oenocytes随后拆除（见议定书第2部分）。同样的程序也可以用于连接到内表面的角质层的其他组织，包括背血管（即心脏）和脂肪体的准备。 请注意，在视频文章oenocyte夹层技术是成年的野生型（广东）男飞，6天的年龄证明。相同的技术可以用于去除雌蝇oenocytes。腹部圆角准备大约...

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在这个视频文章中，我们提出了详细的解剖协议，准备从成人oenocytes D. 果蝇的方式适用于分子生物学分析。其他地方2，解剖方法的一个缩写文本为基础的帐户已被描述和oenocyte准备已被证明是适合提取的RNA和蛋白 2 。使用该制剂，它可能也有可能发展explanted oenocytes培养方法。 oenocyte植体文化协议将有助于调查目的是，确定这些细胞的代谢能力的自主的合成和分泌的?...

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dissection of oenocytes from adult drosophila melanogaster

在<em&gt;果蝇</em&gt;，在其他昆虫一样，上外表面的角质层的蜡质层，主要由烃类化合物，对干燥和其他环境挑战的保护。几个这些表皮烃（CHC）的化合物也可作为semiochemical信号，并作为调解同一物种的成员之间的信息素通讯，或在某些情况下，不同物种之间，并影响人们的行为。简称为oenocytes专门的细胞被视为CHC合成的主站点。但是，相对鲜为人知的是关于oenocytes参与的生物合成，运输，和沉积的途径，促进CHC输出的调控。鉴于社区卫生中心在昆虫生物学的几个方面，包括化学通讯，干燥性和免疫力，发挥的重​​要作用，重要的是要获得一个更好地理解这些专门的细胞内的分子和基因调控CHC生产。成人oenocytes<em&gt; D。果蝇</em位于腹部包膜，metamerically摆着彩带般沿内各腹节的表皮表面辐射的集群。在这个视频文章中，我们展示了一个夹层技术用于准备从成人的oenocytes<em&gt; D。果蝇</em&gt;。具体来说，我们提供了详细的一步一步的示范（1）如何圆角准备成人<em&gt;果蝇</em&gt;腹部，（2）如何识别oenocytes和辨别其他组织，和（3）如何从腹部包膜完好oenocyte集群。包含一个简短的如何该制剂可用于检查碳氢合成有关的基因表达的实验说明。解剖的准备表明此处将允许在成年果蝇的oenocyte功能的详细的分子和遗传分析。

Watch this Scientific Journal Video about Dissection of Oenocytes from Adult Drosophila melanogaster at JoVE.com

Dissection of Oenocytes from Adult Drosophila melanogaster (Video) | JoVE

在昆虫中，oenocytes产生表皮碳氢化合物。这些化合物防止干燥和促进化学通讯。在这里，我们展示了一个夹层的技术，用来隔离从成人oenocytes<em&gt;果蝇</em&gt;，并说明如何可以利用该制剂的研究碳氢合成有关的基因。

从成人Oenocytes剖析果蝇</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

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Dissection of Oenocytes from Adult Drosophila melanogaster

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