Name: in vivo near infrared fluorescence (nirf) intravascular molecular imaging of inflammatory plaque, a multimodal approach to imaging of atherosclerosis
Uploaded: 2011-08-04T12:00:00
Description: Watch this Scientific Journal Video about In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis at JoVE.co

为支持这项工作是由国家卫生授予研究院提供＃108229 R01红莲，美国心脏协会的科学家发展津贴＃0830352N，霍华德休斯医学研究所的职业发展奖，雅涛风险投资公司，欧洲共同体的第七框架计划（下授予FP7/2007-2013协议＃235689），麻省总医院的威廉Schreyer奖学金。

在体内动物模型实验AortoIliac动脉粥样硬化的代 1）基线血管造影和球囊剥脱<ol><li>获得基线血管造影和球囊剥脱之前，新西兰白兔喂食高胆固醇（1％）1周的饮食。这种动物是利用平移的相关性为1）在家兔的主动脉iliacs血管是人类冠状动​​脉（2.5 - 3.5毫米）和2）相同口径的高脂血症，球囊损伤模型，生成类似的炎性细胞（巨噬细胞发炎的动脉粥样硬化的轴承）和分子...

<ol>
	<li>Andersson, J., Libby, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adaptive+immunity+and+atherosclerosis.">Adaptive immunity and atherosclerosis.</a> Clin Immunol. 134, 33-46 (2010).</li><li>Calfon, M. A., Vinegoni, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravascular+near-infrared+fluorescence+molecular+imaging+of+atherosclerosis:+toward+coronary+arterial+visualization+of+biologically+high-risk+plaques.">Intravascular near-infrared fluorescence molecular imaging of atherosclerosis: toward coronary arterial visualization of biologically high-risk plaques.</a> Journal of Biomedical Optics. 15, 011107-011107 (2010).</li><li>Chen, J., Tung, C. -. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+Vivo+Imaging+of+Proteolytic+Activity+in+Atherosclerosis.">In Vivo Imaging of Proteolytic Activity in Atherosclerosis.</a> Circulation. 105, 2766-2771 (2002).</li><li>Jaffer, F. A., Libby, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Imaging+of+Cardiovascular+Disease.">Molecular Imaging of Cardiovascular Disease.</a> Circulation. 116, 1052-1061 (2007).</li><li>Jaffer, F. A., Libby, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+and+Multimodality+Molecular+Imaging:+Insights+Into+Atherosclerosis.">Optical and Multimodality Molecular Imaging: Insights Into Atherosclerosis.</a> Arterioscler Thromb Vasc Biol. 29, 1017-1024 (2009).</li><li>Jaffer, F. A., Vinegoni, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-Time+Catheter+Molecular+Sensing+of+Inflammation+in+Proteolytically+Active+Atherosclerosis.">Real-Time Catheter Molecular Sensing of Inflammation in Proteolytically Active Atherosclerosis.</a> Circulation. 118, 1802-1809 (2008).</li><li>Kim, D. -. E., Kim, J. -. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protease+Imaging+of+Human+Atheromata+Captures+Molecular+Information+of+Atherosclerosis,+Complementing+Anatomic+Imaging.">Protease Imaging of Human Atheromata Captures Molecular Information of Atherosclerosis, Complementing Anatomic Imaging.</a> Arterioscler Thromb Vasc Biol. 30, 449-456 (2010).</li><li>Libby, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflammation+in+atherosclerosis.">Inflammation in atherosclerosis.</a> Nature. 420, 868-874 (2002).</li><li>Naghavi, M., Libby, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+Vulnerable+Plaque+to+Vulnerable+Patient:+A+Call+for+New+Definitions+and+Risk+Assessment+Strategies:+Part+I.">From Vulnerable Plaque to Vulnerable Patient: A Call for New Definitions and Risk Assessment Strategies: Part I.</a> Circulation. 108, 1664-1672 (2003).</li><li>Weissleder, R., Tung, C. -. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+imaging+of+tumors+with+protease-activated+near-infrared+fluorescent+probes.">In vivo imaging of tumors with protease-activated near-infrared fluorescent probes.</a> Nat Biotech. 17, 375-375 (1999).</li><li>Razansky, R. N., Rosenthal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Near-infrared+fluorescence+catheter+system+for+two-dimensional+intravascular+imaging+in+vivo.">Near-infrared fluorescence catheter system for two-dimensional intravascular imaging in vivo.</a> Optics Express. 18, 11372-11381 (2010).</li><li>Jaffer, F. A., Calfon, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-Dimensional+Intravascular+Near-Infrared+Fluorescence+Molecular+Imaging+of+Inflammation+in+Atherosclerosis+and+Stent-Induced+Vascular+Injury.">Two-Dimensional Intravascular Near-Infrared Fluorescence Molecular Imaging of Inflammation in Atherosclerosis and Stent-Induced Vascular Injury.</a> Journal of the American College of Cardiology. 57, 2516-2526 (2011).</li></ol>

发炎的高风险或易损斑块可能大多数心肌梗死的负责。如斑块识别到出现症状之前预测的结果和指导药物治疗的重要的临床意义。如X线血管造影常规冠脉动脉成像方式通常侧重于管腔狭窄的表征，而不是启发性的高风险，通常非狭窄病变的潜在的生物学概况。血管NIRF分子成像提供了一个心脏导管插入术实验室的翻译方法，包括利用纳米传感器，识别活跃的巨噬细胞内斑块斑块炎症的生物学，细?...

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in vivo near infrared fluorescence (nirf) intravascular molecular imaging of inflammatory plaque, a multimodal approach to imaging of atherosclerosis

血管损伤反应是一个精心策划的炎症反应的巨噬细胞内的血管壁，导致积累，管腔内充满脂质斑块，平滑肌细胞增殖和血管腔逐渐缩小的积累引发。这种易损斑块容易破裂，多数情况下急性心肌梗死的基础的形成。在复杂的分子和细胞炎症级联反应是精心策划的招聘T淋巴细胞和巨噬细胞和内皮细胞和平滑肌细胞的旁分泌的影响。 在动脉粥样硬化的分子成像已经演变成一个重要的临床和研究的工具，允许在体内炎症和其他生物过程的可视化。最近的几个例子证明能够及时发现高风险患者的斑块，并评估在动脉粥样硬化pharmacotherapeutics的影响。虽然数量（特别是MRI和PET）的分子成像方法可以形象大血管，如颈生物方面动脉，冠状动脉成像中存在缺乏选项。2高分辨率光学成像战略的问世，尤其是近红外荧光（NIRF），再加上与激活的荧光探针，提高灵敏度，并导致新的血管的战略发展以改善人类冠状动​​脉粥样硬化的生物成像。 分子成像利用近红外荧光（NIRF）定义的频带宽度的光子源（650-900纳米），的光造影剂或荧光探针交付时，发出近红外窗口，可以检测到的荧光激发光使用适当的排放过滤器和一个高灵敏度的电荷耦合相机。相对于可见光，近红外光深深地渗透到组织，如血红蛋白，血脂和水的内源性光子吸收剂明显减弱，并允许由于在近红外窗口，以减少自体荧光的高目标背景比率。成像在近红外“窗口”可以大幅度提高体内成像的潜力。 2,5 已经很好的研究炎症半胱氨酸蛋白酶激活NIRF探头10，在动脉粥样硬化形成中发挥重要作用。通过降解细胞外基质，半胱氨酸蛋白酶作出重要贡献8动脉粥样硬化的进展和并发症。在斑块组织蛋白酶B活性，特别是半胱氨酸蛋白酶，组织蛋白酶B，高表达和colocalizes与巨噬细胞，在实验小鼠，兔，和人类 atheromata此外3,6,7，可以利用先前描述1体内检测- ð血管内的近红外荧光技术6，结合在一个多聚赖氨酸的聚合物骨架多个近红外荧光（VM110/Prosense750，EX / EM 750/780nm，VisEn医疗，沃，MA）的衍生物组成的纳米传感器与注射剂在基线强分子内淬火，结果10以下有针对性的组织蛋白酶B（已知colocalize斑块巨噬细胞），单独的荧光，如半胱氨酸蛋白酶的酶裂解，导致大幅扩增NIRF信号。利用新颖的二维血管NIRF导管血管内的近红外荧光信号检测，使高清晰度，几何体内发炎斑块组织蛋白酶B活性检测的准确。 在体内分子成像使用导管为基础的2D NIRF成像，而不是以前的1 - D光谱方法的动脉粥样硬化，是一种新型的和有前途的工具，利用增强巨噬细胞丰富的斑块蛋白酶活性检测血管炎症 。11,12以下的研究协议介绍，血管内的2维NIRF导管的使用形象和斑块结构特征，利用斑块生物学的关键环节。这是一个翻译的平台，整合现有的临床成像技术，包括血管造影和血管内超声（IVUS），提供了一个独特新颖的综合多式联运分子成像技术，区分炎症atheromata，并允许在人类大小的冠状动脉血管NIRF信号检测。

Watch this Scientific Journal Video about In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis at JoVE.co

In vivo Near Infrared Fluorescence NIRF Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

我们详细介绍了一个新的近红外荧光（NIRF）导管二维血管内斑块的生物学分子成像<em&gt;在体内</em&gt;。 NIRF导管可以想像报告斑块狂热的激活和有针对性的近红外荧光存在的关键生物过程，如炎症。导管采用临床工程和电力的需求和目标是应用在人体冠状动脉。以下的研究描述了一个利用了一种新型的多模态成像战略<em&gt;在体内</em&gt;血管NIRF导管的形象和量化水解积极发炎兔atheromata炎性斑块。

体内近红外荧光（NIRF）血管内分子成像炎性斑块，多式联运方针成像动脉粥样硬化

The vascular response to injury is a well-orchestrated inflammatory response triggered by the accumulation of macrophages within the vessel wall leading ...

Research

JoVE Journal

Medicine

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis

In vivo Dual Substrate Bioluminescent Imaging

In vivo Dual Substrate Bioluminescent Imaging

在体内的双基体生物光学成像

Our understanding of how and when breast cancer cells transit from established primary tumors to metastatic sites has increased at an exceptional rate ...

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Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice

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It is widely accepted that alterations in vascular shear stress trigger the expression of inflammatory genes in endothelial cells and thereby induce ...

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Pharmacological MRI (phMRI) is a new and promising method to study the effects of substances on brain function that can ultimately be used to unravel ...

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Duchenne muscular dystrophy (DMD) is a severe genetic neuromuscular disorder that affects 1 in 3,500 boys, and is characterized by progressive muscle ...

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Fibered confocal fluorescence in vivo imaging with a fiber optic bundle uses the same principle as fluorescent confocal microscopy. It can excite ...

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一个人力<em&gt;前体内</em&gt;动脉粥样硬化斑块模型研究病变的生物学

Atherosclerosis is a chronic inflammatory disease of the vasculature. There are various methods to study the inflammatory compound in atherosclerotic ...

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

组织鬼怪模拟，以评估乳癌手术潜在的近红外荧光成像中的应用

Inaccuracies in intraoperative tumor localization and evaluation of surgical margin status result in suboptimal outcome of breast-conserving surgery ...

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Mouse models are widely used to study pathogenesis of human diseases and to evaluate diagnostic procedures as well as therapeutic interventions ...

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纳米抗体和基于抗体的验证<em&gt;在体内</em&gt;肿瘤异种移植NIRF成像的小鼠实验使用<em&gt;离体</em&gt;流式细胞仪和显微镜

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence (NIRF) xenograft imaging experiments in mice ...

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Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...