Name: methods for experimental manipulations after optic nerve transection in the mammalian cns
Uploaded: 2011-05-12T14:03:00
Description: Watch this Scientific Journal Video about Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS at JoVE.com

PDK는 CIHR 운영 그랜트 (청소 86,523)에 의해 지원됩니다

1. 외과 기술 <ol><li> 실험 무균 기술을 사용하여 특정 기관의 동물 사용 프로토콜에 따라 수행하여야한다. 살아있는 조직과 접촉으로 오는 악기와 자료 (솔루션, 시험 물질, 추적기, 바늘 등) 동물 복지 및 연구에 대한 잠재적인 부정적 영향에 감염하고 불리한 영향을 방지하기 위해 멸균해야합니다. </li></ol> 2. 마취 <ol><li> 쥐 한 동물 isoflurane 기화기 시스템을 사용 anaesthetized 것...

<ol>
	<li>Bahr, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+or+let+die+-+retinal+ganglion+cell+death+and+survival+during+development+and+in+the+lesioned+adult+CNS.">Live or let die - retinal ganglion cell death and survival during development and in the lesioned adult CNS.</a> Trends Neurosci. 23, 483-4890 (2000).</li><li>Isenmann, S., Kretz, A., Cellerino, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+determinants+of+retinal+ganglion+cell+development,+survival,+and+regeneration.">Molecular determinants of retinal ganglion cell development, survival, and regeneration.</a> Prog Retin Eye Res. 22, 483-543 (2003).</li><li>Koeberle, P. D., Bahr, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+and+guidance+cues+for+regenerating+axons:+where+have+they+gone.">Growth and guidance cues for regenerating axons: where have they gone.</a> J Neurobiol. 59, 162-180 (2004).</li><li>Weishaupt, J. H., Bahr, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Degeneration+of+axotomized+retinal+ganglion+cells+as+a+model+for+neuronal+apoptosis+in+the+central+nervous+system+-+molecular+death+and+survival+pathways.">Degeneration of axotomized retinal ganglion cells as a model for neuronal apoptosis in the central nervous system - molecular death and survival pathways.</a> Restor. Neurol. Neurosci. 19, 1-2 (2001).</li><li>Arai-Gaun, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heme+oxygenase-1+induced+in+muller+cells+plays+a+protective+role+in+retinal+ischemia-reperfusion+injury+in+rats.">Heme oxygenase-1 induced in muller cells plays a protective role in retinal ischemia-reperfusion injury in rats.</a> Invest Ophthalmol Vis Sci. 45, 4226-4232 (2004).</li><li>Bainbridge, J. W., Tan, M. H., Ali, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+therapy+progress+and+prospects:+the+eye.">Gene therapy progress and prospects: the eye.</a> Gene Ther. 13, 1191-1197 (2006).</li><li>Polo, A. D. i. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prolonged+delivery+of+brain-derived+neurotrophic+factor+by+adenovirus-infected+Muller+cells+temporarily+rescues+injured+retinal+ganglion+cells.">Prolonged delivery of brain-derived neurotrophic factor by adenovirus-infected Muller cells temporarily rescues injured retinal ganglion cells.</a> Proc Natl Acad Sci U S A. 95, 3978-3983 (1998).</li><li>Herard, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=siRNA+targeted+against+amyloid+precursor+protein+impairs+synaptic+activity+in+vivo.">siRNA targeted against amyloid precursor protein impairs synaptic activity in vivo.</a> Neurobiol Aging. 27, 1740-1750 (2006).</li><li>Koeberle, P. D., Bahr, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+upregulation+of+GLAST-1+is+an+indirect+antiapoptotic+mechanism+of+GDNF+and+neurturin+in+the+adult+CNS.">The upregulation of GLAST-1 is an indirect antiapoptotic mechanism of GDNF and neurturin in the adult CNS.</a> Cell Death Differ. 15, 471-483 (2008).</li><li>Koeberle, P. D., Gauldie, J., Ball, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+adenoviral-mediated+gene+transfer+of+interleukin-10,+interleukin-4,+and+transforming+growth+factor-beta+on+the+survival+of+axotomized+retinal+ganglion+cells.">Effects of adenoviral-mediated gene transfer of interleukin-10, interleukin-4, and transforming growth factor-beta on the survival of axotomized retinal ganglion cells.</a> Neuroscience. 125, 903-920 (2004).</li><li>Naik, R., Mukhopadhyay, A., Ganguli, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+delivery+to+the+retina:+focus+on+non-viral+approaches.">Gene delivery to the retina: focus on non-viral approaches.</a> Drug Discov Today. 14, 306-315 (2009).</li><li>van Adel, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Delivery+of+ciliary+neurotrophic+factor+via+lentiviral-mediated+transfer+protects+axotomized+retinal+ganglion+cells+for+an+extended+period+of+time.">Delivery of ciliary neurotrophic factor via lentiviral-mediated transfer protects axotomized retinal ganglion cells for an extended period of time.</a> Hum Gene Ther. 14, 103-115 (2003).</li><li>Alexander, J. J., Hauswirth, W. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adeno-associated+viral+vectors+and+the+retina.">Adeno-associated viral vectors and the retina.</a> Adv Exp Med Biol. 613, 121-128 (2008).</li><li>Allocca, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=AAV-mediated+gene+transfer+for+retinal+diseases.">AAV-mediated gene transfer for retinal diseases.</a> Expert Opin Biol Ther. 6, 1279-1294 (2006).</li><li>Surace, E. M., Auricchio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Versatility+of+AAV+vectors+for+retinal+gene+transfer.">Versatility of AAV vectors for retinal gene transfer.</a> Vision Res. 48, 353-359 (2008).</li><li>Garcia-Valenzuela, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Axon-mediated+gene+transfer+of+retinal+ganglion+cells+in+vivo.">Axon-mediated gene transfer of retinal ganglion cells in vivo.</a> J Neurobiol. 32, 111-122 (1997).</li><li>Koeberle, P. D., Wang, Y., Schlichter, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kv1.1+and+Kv1.3+channels+contribute+to+the+degeneration+of+retinal+ganglion+cells+after+optic+nerve+transection+in+vivo.">Kv1.1 and Kv1.3 channels contribute to the degeneration of retinal ganglion cells after optic nerve transection in vivo.</a> Cell Death Differ. 17, 134-144 (2010).</li><li>Kugler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transduction+of+axotomized+retinal+ganglion+cells+by+adenoviral+vector+administration+at+the+optic+nerve+stump:+an+in+vivo+model+system+for+the+inhibition+of+neuronal+apoptotic+cell+death.">Transduction of axotomized retinal ganglion cells by adenoviral vector administration at the optic nerve stump: an in vivo model system for the inhibition of neuronal apoptotic cell death.</a> Gene Ther. 6, 1759-1767 (1999).</li><li>Lingor, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Down-regulation+of+apoptosis+mediators+by+RNAi+inhibits+axotomy-induced+retinal+ganglion+cell+death+in+vivo.">Down-regulation of apoptosis mediators by RNAi inhibits axotomy-induced retinal ganglion cell death in vivo.</a> Brain. 128, 550-558 (2005).</li><li>Leon, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lens+injury+stimulates+axon+regeneration+in+the+mature+rat+optic+nerve.">Lens injury stimulates axon regeneration in the mature rat optic nerve.</a> J Neurosci. 20, 4615-4626 (2000).</li><li>Mansour-Robaey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+ocular+injury+and+administration+of+brain-derived+neurotrophic+factor+on+survival+and+regrowth+of+axotomized+retinal+ganglion+cells.">Effects of ocular injury and administration of brain-derived neurotrophic factor on survival and regrowth of axotomized retinal ganglion cells.</a> Proc Natl Acad Sci U S A. 91, 1632-1636 (1994).</li><li>D'Onofrio, P. M., Magharious, M. M., Koeberle, P. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optic+Nerve+Transection:+A+Model+of+Adult.">Optic Nerve Transection: A Model of Adult.</a> J Vis Exp. , .</li></ol>

시신경의 절개면은 성인 CNS의 신경 세포의 apoptosis의 높은 재현성 모델입니다. 이 원고에서 보여준 실험 조작은 부상 후 RGC apoptosis의 메커니즘 연구를 허용합니다. Intraocular 주사는 망막의 전세계를 타겟하는 데 유용합니다. 그것이 유리 피펫의 끝에있는 렌즈를 다치게하지 않는 것이 중요합니다 같은이 절차는 연습이 필요합니다. 렌즈 손상은 세포 생존과 재생 20, 21을</s...

A correction to the article Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS was made.

Erratum: Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Stereotaxic Frame</td>
<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Rat Gas Mask</td>
<td>&nbsp;</td>
<td>Stoelting, Kopf, WPI</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Anesthesia System</td>
<td>&nbsp;</td>
<td>VetEquip</td>
<td>901806</td>
<td>&nbsp;</td>
<tr>
<td>Isoflurane (PrAErrane)</td>
<td>&nbsp;</td>
<td>Baxter Corp</td>
<td>DIN 02225875</td>
<td>&nbsp;</td>
<tr>
<td>Surgical Microscope</td>
<td>&nbsp;</td>
<td>WPI, Zeiss, Leica</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Alcaine Eye Drops</td>
<td>&nbsp;</td>
<td>Alcon</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Tears Naturale P.M.</td>
<td>&nbsp;</td>
<td>Alcon</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Fine tip Dumont forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11252-00</td>
<td>&nbsp;</td>
<tr>
<td>10 &mu;l Hamilton Syringe (1701RN; 26s/2&#x201D;/2)</td>
<td>&nbsp;</td>
<td>Hamilton Syringe Co.</td>
<td>80030</td>
<td>&nbsp;</td>
<tr>
<td>1/16 inch Compression Fittings</td>
<td>&nbsp;</td>
<td>Hamilton Syringe Co.</td>
<td>55751-01</td>
<td>&nbsp;</td>
<tr>
<td>1/16 inch OD, 0.010 inch ID, PEEK Tubing</td>
<td>&nbsp;</td>
<td>Supelco, Bellefonte, PA</td>
<td>Z226661</td>
<td>&nbsp;</td>
<tr>
<td>Dual RN Glass Coupler</td>
<td>&nbsp;</td>
<td>Hamilton Syringe Co.</td>
<td>55752-01</td>
<td>&nbsp;</td>
<tr>
<td>Mineral Oil Priming Kit: includes syringe, needles, rubber septa</td>
<td>&nbsp;</td>
<td>Hamilton Syringe Co.</td>
<td>PRMKIT</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

methods for experimental manipulations after optic nerve transection in the mammalian cns

망막 신경절 세포 (RGCs)는 CNS의 뉴런되는 시신경을 통해 뇌로 망막의 출력 영상 정보. 시신경은 안구의 궤도 내에서 액세스하고 완전히 전체 인구의 RGC axons를 절단 (axotomized) 그었 수 있습니다. 시신경의 절개면은 성인 CNS 1-4에서 apoptotic 세포의 연결을 죽음의 재현성 모델입니다. 눈 유리체 챔버가 intraocular 주사를 통해 실험 조작을 허용, 망막에 약물 전달 캡슐 역할을하기 때문에이 모델은 특히 매력적입니다. 유리 체액을 통해 화학 물질의 확산들은 전체 인구 RGC에 따라 행동을 보장합니다. 바이러스성 벡터, plasmids 또는 짧은 방해 RNAS는 (siRNAs)도 5-12를 망막 세포를 감염 또는 transfect하기 위해 유리 챔버로 전달하실 수 있습니다. Adeno - 관련 바이러스 (AAV) 벡터의 높은 tropism은 주사 사이트 6, 7, 13-15 가까운 세포의 90 %를 접근 감염 속도와 대상 RGCs에 유용합니다. 또한, RGCs은 선택적으로 그들의 목표 우수한 colliculus 10으로 시신경 16-19 또는 주입 벡터의 컷 끝까지 siRNAs, plasmids, 또는 바이러스성 벡터를 적용하여 transfected 수 있습니다. 이것은 연구자들이 다른 방관자의 뉴런이나 주변 glia에 혼란함을 주죠 효과없이 부상의 연결 인구 apoptotic 메커니즘을 공부하실 수 있습니다. RGC apoptosis는 세포 죽음은 세포가 빠르게 타락한 이후 3~4일의 postaxotomy을 지연 의하여 시간 코스 특성이 있습니다. 이것은 apoptosis에 관련된 경로에 대한 이동 실험 조작에 창을 제공합니다. 직접 가로 시신경 스텀프에서 RGCs를 대상으로 노는 즉시 신경을 절단 후, axotomy의 시간에 수행됩니다. 대조적으로, 물질 intraocular 경로를 통해 전달하는 경우, 그들은 axotomized RGCs에서 apoptosis의 개시를 이전, 이전에 수술이나 수술 후 첫 3 일 이내에 주입 수 있습니다. 현재 기사에서 우리는 시신경의 절개 후 실험 조작을위한 여러 가지 방법을 보여줍니다.

Watch this Scientific Journal Video about Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS at JoVE.com

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS

시신경의 절개면은 성인 CNS 손상의 널리 사용되는 모델입니다. 이 모델은 전 세계적으로 망막을 대상에 직접 망막 신경절 세포의 부상의 연결 인구를 대상으로 실험 조작의 번호를 수행하는 데 이상적입니다.

포유류의 CNS에서 시신경의 절개 후 실험에 대한 노는 방법

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. The optic nerve can be ...

Research

JoVE Journal

Neuroscience

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

시신경의 절개 : 중추 신경계에서 신경 세포의 Apoptosis 성인의 모델

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. 
The optic nerve can be ...

연구

신경과학

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

망막 신경절 세포의 생존을 공부 시신경의 호감 상해 Murine 모델

Injury to the optic nerve can lead to axonal degeneration, followed by a gradual death of retinal ganglion cells (RGCs), which results in irreversible ...

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

말초 신경 손상 후 기능 회복을 평가하기 위해 후각 Ensheathing 세포의 이식

Olfactory ensheathing cells (OECs) are neural crest cells which allow growth and regrowth of the primary olfactory neurons. Indeed, the primary olfactory ...

Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish

모터 신경 절개와 라이브 제브라 피쉬의 아교 세포 행동의 시간 경과 영상

The  nervous system is often described as a hard-wired component of the body even  though it is a considerably fluid organ system that reacts to external ...

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

글루타민산 염과 GABA의 빠른 마이크로 이온 도입 : 시냅틱 통합을 조사하는 유용한 도구

One of the fundamental interests in neuroscience is to understand the integration of excitatory and inhibitory inputs along the very complex structure of ...

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice

마우스의 조영 증강 MRI에 의한 시신경 섬유 무결성의 생체 내 이미징

The rodent visual system encompasses retinal ganglion cells and their axons that form the optic nerve to enter thalamic and midbrain centers, and ...

Spinal Cord Transection in the Larval Zebrafish

애벌레 Zebrafish의 척수 절개

Mammals fail in sensory and motor recovery following spinal cord injury due to lack of axonal regrowth below the level of injury as well as an inability ...

Minimally-invasive Technique for Injection into Rat Optic Nerve

쥐 시신경에 주입을위한 최소 침습 기술

The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central ...

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

실험 시신경의 탈수 초화의 쥐 모델에서 시각 유발 전위 기록

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to ...

Exposure of the Pig CNS for Histological Analysis: A Manual for Decapitation, Skull Opening, and Brain Removal

조직 학적 분석을위한 돼지 CNS의 노출 : 목 베기, 두개골 열기위한 매뉴얼 및 뇌 제거

Pigs have become increasingly popular in large-animal translational neuroscience research as an economically and ethically feasible substitute to ...