Name: a mouse model of in utero transplantation
Uploaded: 2011-01-27T17:00:00
Description: Watch this Scientific Journal Video about A Mouse Model of in Utero Transplantation at JoVE.com

हम हमारे वित्तपोषण स्रोतों को स्वीकार करना चाहते हैं: कैलिफोर्निया इंस्टीट्यूट ऑफ पुनर्योजी चिकित्सा क्लीनिकल फैलो प्रशिक्षण अनुदान के लिए (एक), राष्ट्रीय विज्ञान फाउंडेशन (मेगावाट), Irene Perstein पुरस्कार (TCM), सर्जन के अमेरिकी कॉलेज (TCM), अमेरिकी बाल चिकित्सा सर्जिकल एसोसिएशन ( टीसीएम), और ऑफ डाइम्स (टीसीएम) के मार्च.

1. इंजेक्शन pipettes तैयार <ol><li> जैसे कि कांच विंदुक की जुदाई 15 सेकंड (निर्माता अंशांकन के बारे में निर्देश देखने के) के भीतर होता है विंदुक खींचने जांचना. विंदुक एक घटना जहां यह अलग होगा. </li><li> ऐसी है कि घटना की शु?...

<ol>
	<li>Flake, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+X-linked+severe+combined+immunodeficiency+by+in+utero+transplantation+of+paternal+bone+marrow.">Treatment of X-linked severe combined immunodeficiency by in utero transplantation of paternal bone marrow.</a> N Engl J Med. 335, 1806-1810 (1996).</li><li>Wengler, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In-utero+transplantation+of+parental+CD34+haematopoietic+progenitor+cells+in+a+patient+with+X-linked+severe+combined+immunodeficiency+(SCIDXI).">In-utero transplantation of parental CD34 haematopoietic progenitor cells in a patient with X-linked severe combined immunodeficiency (SCIDXI).</a> Lancet. 348, 1484-1487 (1996).</li><li>Flake, A. W., Zanjani, E. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=in+utero+hematopoietic+stem+cell+transplantation:+ontogenic+opportunities+and+biologic+barriers.">in utero hematopoietic stem cell transplantation: ontogenic opportunities and biologic barriers.</a> Blood. 94, 2179-2191 (1999).</li><li>Merianos, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maternal+alloantibodies+induce+a+postnatal+immune+response+that+limits+engraftment+following+in+utero+hematopoietic+cell+transplantation+in+mice.">Maternal alloantibodies induce a postnatal immune response that limits engraftment following in utero hematopoietic cell transplantation in mice.</a> J Clin Invest. 119, 2590-2600 (2009).</li><li>Peranteau, W. H., Endo, M., Adibe, O. O., Flake, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evidence+for+an+immune+barrier+after+in+utero+hematopoietic-cell+transplantation.">Evidence for an immune barrier after in utero hematopoietic-cell transplantation.</a> Blood. 109, 1331-1333 (2007).</li><li>Kim, H. B., Shaaban, A. F., Yang, E. Y., Liechty, K. W., Flake, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microchimerism+and+tolerance+after+in+utero+bone+marrow+transplantation+in+mice.">Microchimerism and tolerance after in utero bone marrow transplantation in mice.</a> J Surg Res. 77, 1-5 (1998).</li><li>Durkin, E. T., Jones, K. A., Rajesh, D., Shaaban, A. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+chimerism+threshold+predicts+sustained+engraftment+and+NK-cell+tolerance+in+prenatal+allogeneic+chimeras.">Early chimerism threshold predicts sustained engraftment and NK-cell tolerance in prenatal allogeneic chimeras.</a> Blood. 112, 5245-5253 (2008).</li><li>Mackenzie, T. C., Shaaban, A. F., Radu, A., Flake, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engraftment+of+bone+marrow+and+fetal+liver+cells+after+in+utero+transplantation+in+MDX+mice.">Engraftment of bone marrow and fetal liver cells after in utero transplantation in MDX mice.</a> J Pediatr Surg. 37, 1058-1064 (2002).</li><li>Hayashi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mixed+chimerism+following+in+utero+hematopoietic+stem+cell+transplantation+in+murine+models+of+hemoglobinopathy.">Mixed chimerism following in utero hematopoietic stem cell transplantation in murine models of hemoglobinopathy.</a> Exp Hematol. 31, 176-184 (2003).</li><li>Bouchard, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+transgene+expression+in+cardiac+and+skeletal+muscle+following+fetal+administration+of+adenoviral+or+adeno-associated+viral+vectors+in+mice.">Long-term transgene expression in cardiac and skeletal muscle following fetal administration of adenoviral or adeno-associated viral vectors in mice.</a> J Gene Med. 5, 941-950 (2003).</li><li>Meza, N. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rescue+of+pyruvate+kinase+deficiency+in+mice+by+gene+therapy+using+the+human+isoenzyme.">Rescue of pyruvate kinase deficiency in mice by gene therapy using the human isoenzyme.</a> Mol Ther. 17, 2000-2009 (2009).</li><li>MacKenzie, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+transduction+of+liver+and+muscle+after+in+utero+injection+of+lentiviral+vectors+with+different+pseudotypes.">Efficient transduction of liver and muscle after in utero injection of lentiviral vectors with different pseudotypes.</a> Mol Ther. 6, 349-358 (2002).</li><li>MacKenzie, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transduction+of+satellite+cells+after+prenatal+intramuscular+administration+of+lentiviral+vectors.">Transduction of satellite cells after prenatal intramuscular administration of lentiviral vectors.</a> J Gene Med. 7, 50-58 (2005).</li><li>Sabatino, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Persistent+expression+of+hF.IX+After+tolerance+induction+by+in+utero+or+neonatal+administration+of+AAV-1-F.IX+in+hemophilia+B+mice.">Persistent expression of hF.IX After tolerance induction by in utero or neonatal administration of AAV-1-F.IX in hemophilia B mice.</a> Mol Ther. 15, 1677-1685 (2007).</li><li>Mellor, A. L., Munn, D. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunology+at+the+maternal-fetal+interface:+lessons+for+T+cell+tolerance+and+suppression.">Immunology at the maternal-fetal interface: lessons for T cell tolerance and suppression.</a> Annu Rev Immunol. 18, 367-391 (2000).</li><li>Billingham, R. E., Brent, L., Medawar, P. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Actively+acquired+tolerance+of+foreign+cells.">Actively acquired tolerance of foreign cells.</a> Nature. 172, 603-606 (1953).</li><li>Endo, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+transfer+to+ocular+stem+cells+by+early+gestational+intraamniotic+injection+of+lentiviral+vector.">Gene transfer to ocular stem cells by early gestational intraamniotic injection of lentiviral vector.</a> Mol Ther. 15, 579-587 (2007).</li><li>Waddington, S. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Long-term+transgene+expression+by+administration+of+a+lentivirus-based+vector+to+the+fetal+circulation+of+immuno-competent+mice.">Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice.</a> Gene Ther. 10, 1234-1240 (2003).</li><li>Schachtner, S., Buck, C., Bergelson, J., Baldwin, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temporally+regulated+expression+patterns+following+in+utero+adenovirus-mediated+gene+transfer.">Temporally regulated expression patterns following in utero adenovirus-mediated gene transfer.</a> Gene Ther. 6, 1249-1257 (1999).</li></ol>

से अधिक 50 साल पहले, billingham, ब्रेंट, और Medawar चूहों में utero प्रत्यारोपण में इस्तेमाल के लिए विदेशी प्रोटीन के लिए प्रतिरक्षा सहिष्णुता प्रेरित उस समय के बाद से. 16, इस तकनीक के कई रूपों इम्यूनोलॉजी और स?...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Pipettes</td>
<td>&nbsp;</td>
<td>Kimble</td>
<td>71900-100</td>
<td>&nbsp;</td>
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<td>Pipette puller</td>
<td>&nbsp;</td>
<td>Sutter Instruments Company</td>
<td>Model P-30</td>
<td>&nbsp;</td>
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<td>Microinjector</td>
<td>&nbsp;</td>
<td>Narishige</td>
<td>IM-300</td>
<td>&nbsp;</td>
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<td>Pipette sharpener</td>
<td>&nbsp;</td>
<td>Sutter Instruments Company</td>
<td>Model BV-10</td>
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a mouse model of in utero transplantation

The transplantation of stem cells and viruses in utero has tremendous potential for treating congenital disorders in the human fetus. For example, in utero transplantation (IUT) of hematopoietic stem cells has been used to successfully treat patients with severe combined immunodeficiency.1,2 In several other conditions, however, IUT has been attempted without success.3 Given these mixed results, the availability of an efficient non-human model to study the biological sequelae of stem cell transplantation and gene therapy is critical to advance this field. We and others have used the mouse model of IUT to study factors affecting successful engraftment of in utero transplanted hematopoietic stem cells in both wild-type mice4-7 and those with genetic diseases.8,9 The fetal environment also offers considerable advantages for the success of in utero gene therapy. For example, the delivery of adenoviral10, adeno-associated viral10, retroviral11, and lentiviral vectors12,13 into the fetus has resulted in the transduction of multiple organs distant from the site of injection with long-term gene expression. in utero gene therapy may therefore be considered as a possible treatment strategy for single gene disorders such as muscular dystrophy or cystic fibrosis. Another potential advantage of IUT is the ability to induce immune tolerance to a specific antigen. As seen in mice with hemophilia, the introduction of Factor IX early in development results in tolerance to this protein.14 
In addition to its use in investigating potential human therapies, the mouse model of IUT can be a powerful tool to study basic questions in developmental and stem cell biology. For example, one can deliver various small molecules to induce or inhibit specific gene expression at defined gestational stages and manipulate developmental pathways. The impact of these alterations can be assessed at various timepoints after the initial transplantation. Furthermore, one can transplant pluripotent or lineage specific progenitor cells into the fetal environment to study stem cell differentiation in a non-irradiated and unperturbed host environment.
The mouse model of IUT has already provided numerous insights within the fields of immunology, and developmental and stem cell biology. In this video-based protocol, we describe a step-by-step approach to performing IUT in mouse fetuses and outline the critical steps and potential pitfalls of this technique.

Watch this Scientific Journal Video about A Mouse Model of in Utero Transplantation at JoVE.com

A Mouse Model of in Utero Transplantation

माउस के मॉडल<em&gt; Utero में</em&gt; प्रत्यारोपण एक बहुमुखी उपकरण है कि भ्रूण में स्टेम सेल और जीन थेरेपी प्रत्यारोपण के संभावित नैदानिक ​​अनुप्रयोगों का अध्ययन करने के लिए इस्तेमाल किया जा सकता है. इस प्रोटोकॉल में, हम इस तकनीक का प्रदर्शन करने के लिए एक सामान्य दृष्टिकोण प्रस्तुत

एक माउस के मॉडल Utero में ट्रांसप्लांटेशन

The transplantation of stem cells and viruses in utero has tremendous potential for treating congenital disorders in the human fetus.  For example, in ...

Research

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Medicine

A Mouse Model of in Utero Transplantation

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Heterotopic हृदय प्रत्यारोपण के एक माउस मॉडल का उपयोग पल्मोनरी वाल्व के प्रत्यारोपण

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Murine प्रत्यारोपण: ठोस अंग प्रत्यारोपण के सबसे आम रूप अध्ययन के लिए एक मॉडल

Corneal transplantation is the most common form of organ transplantation in the United States with between 45,000 and 55,000 procedures performed each ...

Transplantation Into the Mouse Ovarian Fat Pad

माउस डिम्बग्रंथि वसा पैड में प्रत्यारोपण

Orthotopic transplantation assays in mice are invaluable for studies of cell regeneration and neoplastic transformation. Common approaches for orthotopic ...

Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video

एक अनुदेशात्मक वीडियो-महाधमनी प्रत्यारोपण के एक माउस मॉडल में आस्तीन तकनीक का उपयोग करना

Orthotopic aortic transplantation using the sleeve technique reduces injury to the aorta with failure rate of only 10-20%. The time to anastomose the ...

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

अग्ंयाशय प्रत्यारोपण के लिए माउस मॉडल एक संशोधित कफ तकनीक का उपयोग

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the ...

Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse

Intravital माइक्रोस्कोपी और एक हेयरलैस माउस के कान के सिरे में thrombus प्रेरण

Thrombotic complications of vascular diseases are one leading cause of morbidity and mortality in industrial nations. Due to the complex interactions ...

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

एक माउस मॉडल में कार्डियोपल्मोनरी बाईपास: एक उपंयास दृष्टिकोण

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...

Intraportal Transplantation of Pancreatic Islets in Mouse Model

माउस मॉडल में अग्नाशय के टाप का Intraportal प्रत्यारोपण

Pancreatic islet transplantation to reduce hyperglycemia is highly successful in rodents with chemically-induced diabetes. The most common transplantation ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

माउस के Isometric सिकुड़ना माप Mesenteric धमनी का उपयोग तार Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Veno-Venous Extracorporeal Membrane Oxygenation in a Mouse

Veno-एक माउस में शिरापरक Extracorporeal झिल्ली ऑक्सीजन

The use of extracorporeal membrane oxygenation (ECMO) has increased substantially in recent years. ECMO has become a reliable and effective therapy for ...