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DOI :
10.3791/2315-v
December 23rd, 2010
Chapters
0:05
Title
1:13
Introduction
1:48
Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
2:42
Preparation of Lipid Loaded Micropipette
4:20
Live-cell Imaging of Cell Migration in Response to Ligand Application
5:25
Representative Results for Migration of BMDCs
6:03
Live Cell Imaging of Transfected RBL-2H3 Cells
9:49
Image Analysis
10:51
Representative Results
13:03
Conclusion
본 논문은 특정 리간드에 leukocytes의 chemotactic 응답을 확인하고 라이브 셀 이미징 기술을 사용하여 세포 표면 수용체와 cytosolic 단백질 사이의 상호 작용을 식별하는 방법을 설명합니다.
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