Name: real-time imaging of leukotriene b4 mediated cell migration and blt1 interactions with &beta;-arrestin
Uploaded: 2010-12-23T15:00:00
Description: Watch this Scientific Journal Video about Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with ÃŽÂ²-arrestin at JoVE.com

A pesquisa é suportada pelo National Institutes of Health bolsas AI-52381, CA138623 e Lung Cancer Research Board Kentucky e apoio institucional de James Graham Brown Cancer Center.

Metodologia Descrição do Microscópio Experimentos com imagens ao vivo celular realizado TE-FM sistema Epi-Fluorescência anexado ao microscópio invertido Nikon Eclipse TE300. O microscópio equipado com estágio de aquecimento. A HQ digital de pressão fresca B / W CCD (Roper Scientific) câmera e LAMDA 02/10 óptico filtro changer (Sutter empresa instrumento) é anexado ao microscópio. Comprimentos de onda de excitação e emissão são controlados com rodas de ...

<ol>
	<li>Wess, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=G-protein-coupled+receptors:+molecular+mechanisms+involved+in+receptor+activation+and+selectivity+of+G-protein+recognition.">G-protein-coupled receptors: molecular mechanisms involved in receptor activation and selectivity of G-protein recognition.</a> FASEB J. 11, 346-354 (1997).</li><li>Gether, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Uncovering+molecular+mechanisms+involved+in+activation+of+G+protein-coupled+receptors.">Uncovering molecular mechanisms involved in activation of G protein-coupled receptors.</a> Endocr Rev. 21, 90-113 (2000).</li><li>Pierce, K. L., Premont, R. T., Lefkowitz, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Seven-transmembrane+receptors.">Seven-transmembrane receptors.</a> Nat Rev Mol Cell Biol. 3, 639-650 (2002).</li><li>Lefkowitz, R. J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=protein-coupled+receptors.+III.+New+roles+for+receptor+kinases+and+beta-arrestins+in+receptor+signaling+and+desensitization.">protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization.</a> J Biol Chem. 273, 18677-18680 (1998).</li><li>Shenoy, S. K., Lefkowitz, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multifaceted+roles+of+beta-arrestins+in+the+regulation+of+seven-membrane-spanning+receptor+trafficking+and+signalling.">Multifaceted roles of beta-arrestins in the regulation of seven-membrane-spanning receptor trafficking and signalling.</a> Biochem J. 375, 503-515 (2003).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beta-arrest	or.">Beta-arrest	or.</a> Nature. 383, 447-450 (1996).</li><li>Serhan, C. N., Haeggstrom, J. Z., Leslie, C. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipid+mediator+networks+in+cell+signaling:+update+and+impact+of+cytokines.">Lipid mediator networks in cell signaling: update and impact of cytokines.</a> Faseb J. 10, 1147-1158 (1996).</li><li>Tager, A. M., Luster, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=BLT1+and+BLT2:+the+leukotriene+B(4)+receptors.">BLT1 and BLT2: the leukotriene B(4) receptors.</a> Prostaglandins Leukot Essent Fatty Acids. 69, 123-134 (2003).</li><li>Toda, A., Yokomizo, T., Shimizu, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Leukotriene+B4+receptors.">Leukotriene B4 receptors.</a> Prostaglandins Other Lipid Mediat. 68-69, 575-585 (2002).</li><li>Haribabu, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+disruption+of+the+leukotriene+B(4)+receptor+in+mice+reveals+its+role+in+inflammation+and+platelet-activating+factor-induced+anaphylaxis.">Targeted disruption of the leukotriene B(4) receptor in mice reveals its role in inflammation and platelet-activating factor-induced anaphylaxis.</a> J Exp Med. 192, 433-438 (2000).</li><li>Subbarao, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+leukotriene+B4+receptors+in+the+development+of+atherosclerosis:+potential+mechanisms.">Role of leukotriene B4 receptors in the development of atherosclerosis: potential mechanisms.</a> Arterioscler Thromb Vasc Biol. 24, 369-375 (2004).</li><li>Jala, V. R., Haribabu, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Leukotrienes+and+atherosclerosis:+new+roles+for+old+mediators.">Leukotrienes and atherosclerosis: new roles for old mediators.</a> Trends Immunol. 25, 315-322 (2004).</li><li>Heller, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+atherogenesis+in+BLT1-deficient+mice+reveals+a+role+for+LTB4+and+BLT1+in+smooth+muscle+cell+recruitment.">Inhibition of atherogenesis in BLT1-deficient mice reveals a role for LTB4 and BLT1 in smooth muscle cell recruitment.</a> Circulation. 112, 578-586 (2005).</li><li>Miyahara, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Requirement+for+leukotriene+B4+receptor+1+in+allergen-induced+airway+hyperresponsiveness.">Requirement for leukotriene B4 receptor 1 in allergen-induced airway hyperresponsiveness.</a> Am J Respir Crit Care Med. 172, 161-167 (2005).</li><li>Terawaki, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Absence+of+leukotriene+B4+receptor+1+confers+resistance+to+airway+hyperresponsiveness+and+Th2-type+immune+responses.">Absence of leukotriene B4 receptor 1 confers resistance to airway hyperresponsiveness and Th2-type immune responses.</a> J Immunol. 175, 4217-4225 (2005).</li><li>Shao, W. H., Del Prete, A., Bock, C. B., Haribabu, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+disruption+of+leukotriene+B4+receptors+BLT1+and+BLT2:+a+critical+role+for+BLT1+in+collagen-induced+arthritis+in+mice.">Targeted disruption of leukotriene B4 receptors BLT1 and BLT2: a critical role for BLT1 in collagen-induced arthritis in mice.</a> J Immunol. 176, 6254-6261 (2006).</li><li>Kim, N. D., Chou, R. C., Seung, E., Tager, A. M., Luster, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+unique+requirement+for+the+leukotriene+B4+receptor+BLT1+for+neutrophil+recruitment+in+inflammatory+arthritis.">A unique requirement for the leukotriene B4 receptor BLT1 for neutrophil recruitment in inflammatory arthritis.</a> J Exp Med. 203, 829-835 (2006).</li><li>Jala, V. R., Shao, W. H., Haribabu, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phosphorylation-independent+beta-arrestin+translocation+and+internalization+of+leukotriene+B4+receptors.">Phosphorylation-independent beta-arrestin translocation and internalization of leukotriene B4 receptors.</a> J Biol Chem. 280, 4880-4887 (2005).</li><li>Jala, V. R., Haribabu, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+analysis+of+G+protein-coupled+receptor+signaling+in+live+cells.">Real-time analysis of G protein-coupled receptor signaling in live cells.</a> Methods Mol Biol. 332, 159-165 (2006).</li><li>Del Prete, A., A, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+dendritic+cell+migration+and+adaptive+immune+response+by+leukotriene+B4+receptors:+a+role+for+LTB4+in+up-regulation+of+CCR7+expression+and+function.">Regulation of dendritic cell migration and adaptive immune response by leukotriene B4 receptors: a role for LTB4 in up-regulation of CCR7 expression and function.</a> Blood. 109, 626-631 (2007).</li><li>Salogni, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activin+A+induces+dendritic+cell+migration+through+the+polarized+release+of+CXC+chemokine+ligands+12+and+14.">Activin A induces dendritic cell migration through the polarized release of CXC chemokine ligands 12 and 14.</a> Blood. 113, 5848-5856 (2009).</li><li>Boudreau, J., Koshy, S., Cummings, D., Wan, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Culture+of+myeloid+dendritic+cells+from+bone+marrow+precursors.">Culture of myeloid dendritic cells from bone marrow precursors.</a> J Vis Exp. , (2008).</li></ol>

Imagens de células vivas é uma poderosa ferramenta para demonstrar a função e interações de proteínas específicas à medida que ocorrem em tempo real. Os métodos descritos neste manuscrito mostram claramente que a LTB 4 pode induzir a migração rápida de células dendríticas. Estes métodos não só ampliar os aspectos da função LTB 4 a diversos tipos de células, eles permitem que métodos semelhantes para ser aplicado a uma variedade de quimiocinas e outros testes de sua eficácia c...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Cell lines:</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Rat Basophilic Leukomia Cell line (RBL-2H3) or HEK293 cells.</td>
<td>&nbsp;</td>
<td>ATCC</td>
<td>CRL-2256</td>
<td>&nbsp;</td>
<tr>
<td>Media:</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Delbecco's modified Eagle&#x2019;s Medium (DMEM)</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>11995</td>
<td>&nbsp;</td>
<tr>
<td>Phenol red free RPMI or DMEM</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>11835-030</td>
<td>&nbsp;</td>
<tr>
<td>Fetal Bovine Serum</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>16000-044</td>
<td>&nbsp;</td>
<tr>
<td>L-Glutamine (200 mM)</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>25030</td>
<td>&nbsp;</td>
<tr>
<td>Penicillin-streptomycin (10000 U/mL)</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>15140</td>
<td>&nbsp;</td>
<tr>
<td>Trypsin, 0.05% (1X) with EDTA 4Na, liquid</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>25300</td>
<td>&nbsp;</td>
<tr>
<td>HEPES (1M)</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>15630</td>
<td>&nbsp;</td>
<tr>
<td>Others:</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>35 mm sterile glass coverslip-bottomed Fluoro dishes (0.17 mm thick) (WillCo-dish)</td>
<td>&nbsp;</td>
<td>WPI</td>
<td>FD35-100</td>
<td>&nbsp;</td>
<tr>
<td>Sterile Gene Pulser Cuvette (0.4 cm electrode gap) (Bio-Rad)</td>
<td>&nbsp;</td>
<td>Bio-Rad</td>
<td>16552088</td>
<td>&nbsp;</td>
<tr>
<td>Instruments/software:</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Gene Pulser II electroporater </td>
<td>&nbsp;</td>
<td>Bio-Rad</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TE-FM Epi-Fluorescence system attached to Nikon Inverted Microscope Eclipse TE300</td>
<td>&nbsp;</td>
<td>Nikon</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Metamorph Software</td>
<td>&nbsp;</td>
<td>Universal Imaging</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Vertical Micro-pipette puller</td>
<td>&nbsp;</td>
<td>Narishige International </td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Micro-Forge M-900</td>
<td>&nbsp;</td>
<td>Narishige International </td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Hadraulic Micromanipulator MO-188NE</td>
<td>&nbsp;</td>
<td>Narishige International </td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Coarse Manual Manipulator, MN-188NE</td>
<td>&nbsp;</td>
<td>Narishige International </td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>cDNA constructs:</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>cDNA of G-Protein coupled receptor tagged with red fluorescence protein at C-terminus (hBLT1-RFP)</td>
<td>&nbsp;</td>
<td>Jala et al 2005</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td> cDNA of cytosolic protein tagged with GFP (&beta;-arrestin1-GFP in present study).</td>
<td>&nbsp;</td>
<td>Jala et al 2005</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

real-time imaging of leukotriene b4 mediated cell migration and blt1 interactions with &beta;-arrestin

Receptores acoplados à proteína G (GPCRs) pertencem à família de proteínas transmembrana sete e mediar a transdução de sinais extracelulares de respostas intracelulares. GPCRs controle de diversas funções biológicas, como quimiotaxia, liberação de cálcio intracelular, regulação de genes de uma forma ligante dependente via heterotrimeric G-proteínas 1-2. Ligante de ligação induz uma série de mudanças conformacionais levando à ativação de heterotrimeric G-proteínas que modulam níveis de segundos mensageiros como a adenosina monofosfato cíclico (cAMP), o trifosfato de inositol (IP3) e glicerol diacyl (DG). Concomitante com a ativação da ligação ligante receptor também inicia uma série de eventos para atenuar o receptor de sinalização por meio de seqüestro de dessensibilização, e / ou internalização. O processo de dessensibilização dos GPCRs ocorre através de fosforilação do receptor de quinases da proteína G-receptor (GRKs) e subsequente ligação dos β-arrestinas 3. β-arrestinas são proteínas citosólicas e translocar para a membrana após a ativação GPCR, ligação aos receptores fosforilados (maioria dos casos) há, facilitando a internalização dos receptores 4-6. Leucotrieno B 4 (LTB 4) é uma molécula de lipídio pró-inflamatórias derivadas de ácido araquidônico via e medeia suas ações através de GPCRs, LTB 4 receptor 1 (BLT1; um receptor de alta afinidade) e receptor LTB 4 2 (BLT2; um receptor de baixa afinidade ) 7-9. A LTB via 4-BLT1 foi mostrado para ser crítico em várias doenças inflamatórias, incluindo, artrite, asma e aterosclerose 10-17. O presente artigo descreve as metodologias desenvolvidas para monitorar LTB 4 induzida migração de leucócitos e as interações de BLT1 com β-arrestina e, a translocação do receptor em células vivas usando técnicas de microscopia de imagem 18-19. Derivadas da medula óssea células dendríticas de camundongos C57BL / 6 foram isoladas e cultivadas como descrito anteriormente 20-21. Essas células foram testados em métodos de imagem ao vivo de células para demonstrar LTB 4 migração celular induzida. O BLT1 humano foi marcado com uma proteína fluorescente vermelha (BLT1-RFP) no C-terminal e β-arrestin1 marcados com proteína fluorescente verde (β-arr-GFP) e os plasmídeos transfectados em ambos os Rat basofílico Leukomia (RBL-2H3) linhas celulares 18-19. A cinética de interação entre essas proteínas e localização foram monitorados através de microscopia de vídeo ao vivo da célula. As metodologias no presente trabalho descrevem o uso de técnicas microscópicas para investigar as respostas funcionais do G receptores acoplados à proteína em células vivas. O presente artigo também descreve o uso de Metamorph software para quantificar a intensidade de fluorescência para determinar a cinética do receptor e interações proteína citosólica.

Watch this Scientific Journal Video about Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with ÃŽÂ²-arrestin at JoVE.com

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with ÃƒÅ½Ã‚Â²-arrestin

Este artigo descreve a metodologia para determinar a resposta quimiotática de leucócitos para ligantes específicos e identificar as interações entre os receptores da superfície celular e as proteínas citosólicas usando técnicas de imagem de células vivas.

Em tempo real de imagens de leucotrienos B 4 Migração mediada por células e BLT1 Interações com β-arrestina

G-protein coupled receptors (GPCRs) belong to the seven transmembrane protein family and mediate the transduction of extracellular signals to ...

Research

JoVE Journal

Immunology and Infection

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with &beta;-arrestin

Real-time Live Imaging of T-cell Signaling Complex Formation

Em tempo real de imagens ao vivo de células T Sinalização de Formação de Complexos

Protection against infectious diseases is mediated by the immune system 1,2. T lymphocytes are the master coordinators of the immune system, regulating ...

Imunologia e Infecção

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoramento da Migração de Células dendríticas usando<sup&gt; 19</sup&gt; F /<sup&gt; 1</sup&gt; H Ressonância Magnética

Continuous  advancements in noninvasive imaging modalities such as magnetic resonance  imaging (MRI) have greatly improved our ability to study ...

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Imagens em tempo real de heterotípica plaquetas neutrófilos Interações sobre o endotélio ativado durante a inflamação vascular e formação de trombos em camundongos vivos

Interaction of activated platelets and leukocytes (mainly neutrophils) on the activated endothelium mediates thrombosis and vascular inflammation.1,2 ...

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Imagens em tempo real de endoteliais junções célula-célula durante Neutrophil Transmigração Sob Fisiológica Fluxo

During inflammation, leukocytes leave the circulation and cross the endothelium to fight invading pathogens in underlying tissues. This process is known ...

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Imagens em tempo real de células mielóides em Dynamics<em&gt; Apc<sup&gt; Min / +</sup</em&gt; Intestinal Tumores girando Disk microscopia confocal

Myeloid cells are the most abundant immune cells within tumors and have been shown to promote tumor progression. Modern intravital imaging techniques ...

Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis

Migração intersticial imagens de células CD4 T na derme inflamadas

The ability of CD4 T cells to carry out effector functions is dependent upon the rapid and efficient migration of these cells in inflamed peripheral ...

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

High Throughput, em tempo real, Teste Dual-leitura dos intracelular Actividade Antimicrobiana e citotoxicidade celular eucariótica

Traditional measures of intracellular antimicrobial activity and eukaryotic cell cytotoxicity rely on endpoint assays. Such endpoint assays require ...

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System

Imagem em tempo real e quantificação de fungos biofilme desenvolvimento usando um sistema bifásico de fluxo de recirculação

In oropharyngeal candidiasis, members of the genus Candida must adhere to and grow on the oral mucosal surface while under the effects of salivary flow. ...

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

Modelo de doença microfluida in vitro para estudar interações endoteliais de sangue inteiro e dinâmica do coágulo sanguíneo em tempo real

The formation of blood clots involves complex interactions between endothelial cells, their underlying matrix, various blood cells, and proteins. The ...

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Monitorando a sobrevivência do vírus da influenza fora do hospedeiro usando análise celular em tempo real

Methods for virus particle quantification represent a critical aspect of many virology studies. Although several reliable techniques exist, they are ...