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DOI :
10.3791/2316-v
•
10:46 min
December 14th, 2010
Chapters
0:00
Title
0:57
Introduction
3:36
Isolating Mouse Pulmonary Endothelial Cells from Neonatal Mice
1:58
Preparing Anti-PECAM-1 Antibody-Conjugated Magnetic Beads
6:56
Sorting Mouse Lung Endothelial Cells with Anti-ICAM-2 Dynabeads
9:57
Conclusion
9:13
Evaluation of Pulmonary Endothelial Cells from Neonatal Mice
在这里,我们描述了一个小鼠肺血管内皮细胞的分离和培养协议。这种方法包括机械和酶的肺组织分解以及2步纯化过程中使用抗PECAM - 1和抗ICAM - 2的抗体结合磁珠,这将产生一个纯粹的原籍大多微血管的内皮细胞的人口。
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