Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/2316-v
•
10:46 min
December 14th, 2010
Chapters
0:00
Title
0:57
Introduction
3:36
Isolating Mouse Pulmonary Endothelial Cells from Neonatal Mice
1:58
Preparing Anti-PECAM-1 Antibody-Conjugated Magnetic Beads
6:56
Sorting Mouse Lung Endothelial Cells with Anti-ICAM-2 Dynabeads
9:57
Conclusion
9:13
Evaluation of Pulmonary Endothelial Cells from Neonatal Mice
ここで、我々は、マウス肺内皮細胞の単離と培養のためのプロトコルについて説明します。このメソッドは、メカニックと酵素肺組織の解離と同様に抗PECAM - 1とほとんどが微小血管起源の純粋な内皮細胞集団を生成する磁気ビーズへの結合抗ICAM - 2抗体を用いた2ステップ精製プロセスを含む。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved