Name: modeling biological membranes with circuit boards and measuring electrical signals in axons: student laboratory exercises
Uploaded: 2011-01-18T18:00:00
Duration: 13 min 56 s
Description: Watch this Scientific Journal Video about Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises at JoVE.com

从一门课程，已在使用的实验室手册，策划博士HL阿特伍德在多伦多大学动物学系，这些实验进行了修改。这次演习也被用来从制作“ 第 6 次密集IBRO基本神经科学研讨会”，并在1993年（Cooper 等人，1993年），汉城，韩国高丽大学举行的手动修改。目前的修改，需要共同使用的设备到目前在各大学的一天学生定向实验室。肯塔基大学生物系，本科课程和艺术与科学学院办公室支持。

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我们在上线的视频演示，本文的目的是证明细胞的生物物理特性，可以部分，作为电子电路建模。此外，活的神经组织，是比较容易获得，传导速度，不应期和电生理记录技术的基本原则可能与温和的设备投资本科学生实验室。的主题和提出的基本范式，可以很容易地修改为不同课程的要求。 小龙虾和它的丰富的维护，以学生为主导的试验有吸引力的车型。甲壳动物腹神经线一般坚固，并保留最少一个小时的盐水，这是3个小时的学生实验室有足够的生理完整性。 鉴于一些小龙虾的VNC大轴突通过缝隙连接，可以进行额外的实验上所作出的贡献，以及不同的特性，比标准蛙坐骨神经准备发现可证明连接。坐骨神经是一个经典模型，为解决复合动作电位传导性能。它甚至可能是一个有趣的对比实验，让学生比较传导性能，轴突招聘，这两种制剂之间不应期。

电路板 <ol><li>下面列出的实验电路板的实验电子，可在当地的电子产品，如Radio Shack的存储。 </li><li>面包板，电阻，电容，电池和导线，作为实验中的实验电路板的路口。 </li><li>一个共同的电压表（Wavetek Meterman电压表） </li><li> A / D板线录制到计算机。电信号记录到PowerLab/4s接口（ADInstruments，CO，美国科罗拉多州的斯普林斯，）上线。我们使用标准软件名为图表或范围ADInstruments。 </li></ol> 生理学实验 <ol><li>小龙虾（ 克氏螯虾 ）。阿查法拉亚生物供应公司，Raceland，洛杉矶，美国。 </li><li>小龙虾生理盐水（标准）：范Harreveld的解决方案（1936年）修改。 （205毫米）氯化钠氯化钾5.3; 13.5氯化钙2 · 2H 2 O; 2.45氯化镁2 · 6H 2 O 5 HEPES和调整pH至7.4。从Sigma公司（圣路易斯，密苏里州）获得所有生理盐水化学品。 </li><li>解剖工具：精细＃5镊子，精剪，刀刀座，昆虫引脚＃26002-20（所有获得精细科学的工具（美国）公司，373 - G的复古公园径，福斯特市，CA 94404-1139） </li><li>一个神经腔菜（ADInstruments，CO，美国科罗拉多州的斯普林斯，）举行的神经。可用于刺激神经与神经腔菜来的电线。 </li><li>吸电极是用来记录的信号。 </li><li>操纵或钳上环站也将作为持有吸电极。 </li><li>法拉第笼</li><li>解剖显微镜</li><li>高强度照明灯（光源） </li><li>显微镜平台</li><li>差分放大器的AC / DC（AM系统公司型号3000） </li><li> PowerLab系统26T（公元仪器） </li><li>外放大器（公元仪器） </li><li> LabChart 7（ADI仪器） </li><li>解剖工具</li><li> A环的立场和钳作为持有录音吸电极</li></ol>

modeling biological membranes with circuit boards and measuring electrical signals in axons: student laboratory exercises

这是一个示范如何电气模型可以用来描述生物膜。这次演习还介绍了中电使用的生物物理术语。膜模型使用相同的设备在现场准备工作。调查的一个孤立的神经线的一些属性：神经的动作电位，神经元的招聘，和环境因素的反应神经线。

Watch this Scientific Journal Video about Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises at JoVE.com

Modeling Biological Membranes with Circuit Boards and Measuring Electrical Signals in Axons: Student Laboratory Exercises

这是一个示范如何使用电气模型，是可以理解的生物膜。我们还演示程序，记录从学生为本的实验室小龙虾的腹神经索的动作电位。

建模生物膜与电路板，并在轴突测量电气信号：学生实验室练习

This is a demonstration of how electrical models can be used to characterize biological membranes. This exercise also introduces biophysical terminology ...

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