Name: using bioluminescent imaging to investigate synergism between streptococcus pneumoniae and influenza a virus in infant mice
Uploaded: 2011-04-14T11:00:00
Duration: 10 min 47 s
Description: Watch this Scientific Journal Video about Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice at JoVE.com

作者要感谢大卫Briles（在伯明翰，AL，美国阿拉巴马大学）的技术咨询。我们想感谢有益的建议和讨论关于动物福利与伦理和干预的标准和人性化的终点的发展所描述的实验伊薇特陈，墨尔本大学的动物福利主任，和大卫泰勒，动物设施经理，在这个手稿。 Odilia Wijburg和帕特里克读由NHMRC RD莱特奖学金的支持，科斯蒂短是由葛兰素史克研究生支援津贴和Puzey奖学金支持。

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	<li>Brundage, J. F., Shanks, G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deaths+from+bacterial+pneumonia+during+1918-19+influenza+pandemic.">Deaths from bacterial pneumonia during 1918-19 influenza pandemic.</a> Emerg Infect Dis. 14, 1193-1199 (2008).</li><li>Petersdorf, R. G., Fusco, J. J., Harter, D. H., Albrink, W. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+infections+complicating+Asian+influenza.">Pulmonary infections complicating Asian influenza.</a> AMA Arch Intern Med. 103, 262-272 (1959).</li><li>Diavatopoulos, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influenza+A+virus+facilitates+Streptococcus+pneumoniae+transmission+and+disease.">Influenza A virus facilitates Streptococcus pneumoniae transmission and disease.</a> Faseb J. , (2010).</li><li>Orihuela, C. J., Gao, G., Francis, K. P., Yu, J., Tuomanen, E. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue-specific+contributions+of+pneumococcal+virulence+factors+to+pathogenesis.">Tissue-specific contributions of pneumococcal virulence factors to pathogenesis.</a> J Infect Dis. 190, 1661-1669 (2004).</li><li>Kadurugamuwa, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+monitoring+of+pneumococcal+meningitis+and+evaluation+of+treatment+efficacy+in+an+experimental+mouse+model.">Noninvasive monitoring of pneumococcal meningitis and evaluation of treatment efficacy in an experimental mouse model.</a> Mol Imaging. 4, 137-142 (2005).</li><li>Smith, M. W., Schmidt, J. E., Rehg, J. E., Orihuela, C. J., McCullers, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+pro-+and+anti-inflammatory+molecules+in+a+mouse+model+of+pneumococcal+pneumonia+after+influenza.">Induction of pro- and anti-inflammatory molecules in a mouse model of pneumococcal pneumonia after influenza.</a> Comp Med. 57, 82-89 (2007).</li><li>Owen, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nasal-associated+lymphoid+tissue+and+olfactory+epithelium+as+portals+of+entry+for+Burkholderia+pseudomallei+in+murine+melioidosis.">Nasal-associated lymphoid tissue and olfactory epithelium as portals of entry for Burkholderia pseudomallei in murine melioidosis.</a> J Infect Dis. 199, 1761-1770 (2009).</li><li>Luker, G. D., Leib, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Luciferase+real-time+bioluminescence+imaging+for+the+study+of+viral+pathogenesis.">Luciferase real-time bioluminescence imaging for the study of viral pathogenesis.</a> Methods Mol Biol. 292, 285-296 (2005).</li><li>Hutchens, M., Luker, G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Applications+of+bioluminescence+imaging+to+the+study+of+infectious+diseases.">Applications of bioluminescence imaging to the study of infectious diseases.</a> Cell Microbiol. 9, 2315-2322 (2007).</li></ol>

生产这种视频文章赞助卡尺生命科学。

IVIS机体内成像是一种独特的方法，使实时的可视化的微生物的发病 4 ，6-9。在这里，我们展示了这项技术的应用，以了解南之间的协同作用婴儿小鼠肺炎和流感病毒。由于这项技术的非侵入性的性质，我们已经能够监测感染的进展在每一个人随着时间的推移鼠标。这使我们共同居住的婴儿小鼠 3文件之间的肺炎球菌传播的动力学。这项技术的非侵入性使研究人员能够使用较少，每实验动物，从而减少动物的使用。它也可以使用IVIS机，可视化的传播感染，在体内感染以前未知的网站，以及不容易被清扫（如中耳）采样地点。 成像实验开始之前，我们证实，发光EF3030应变的殖民化水平与殖民父EF3030列举组织匀浆活菌计数的应变水平。此外，我们首次证实流感病毒感染导致发光EF3030负荷增加鼻咽殖民父EF3030株（结果未显示）表明动物。 IVIS技术虽然易于操作，并产生重现性好，易于理解的数据，实验必须精心设计，使该技术的敏感性和实用的最大化。例如，IVIS相机的灵敏度是影响的深度和不透明的组织8，这在我们的经验，增加的检测限为来自肺部的发光信号。此外，深色皮毛和皮肤色素减少高达10倍（无毛小鼠相比）8的光传输。发展这种方法的同时，我们也使用5至14日龄C57BL / 6小鼠进行了优化本议定书，并自表明，IVIS可以用来检测20日龄C57BL / 6小鼠的定植与发光S. 肺炎 EF3030。然而，信号检测，这些裸体或BALB / c小鼠进行实验时，可能会增加。然而，IVIS相机代表了一种新的方法来监测，鉴定和最终理解A型流感病毒感染后在体内的肺炎球菌病的发展。

using bioluminescent imaging to investigate synergism between streptococcus pneumoniae and influenza a virus in infant mice

在1918年的流感病毒大流行，全球约50亿人死亡，死亡的多数是不单独与流感病毒感染的结果。相反，大多数人认为死于继发性细菌感染，他们主要由肺炎链球菌 （肺炎球菌）引起的。由流感病毒和肺炎球菌引起的感染之间的协同关系，随后被观察到在1957年的亚洲流感病毒大流行，以及在季节性暴发的病毒（1，2审查）。在这里，我们描述机制（S）作为并发A型流感病毒和S.增加发病率可能会在参与调查的协议肺炎衣原体感染。我们已经开发出一个婴儿的小鼠模型可靠和可重复的展示与S殖民统治的小鼠流感病毒感染的影响肺炎 。使用此协议，我们提供了共同安置在体内成像3，新生小鼠肺炎球菌之间的传输动力学的第一洞察力。

Watch this Scientific Journal Video about Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice at JoVE.com

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

与A型流感病毒并发感染是牵连在无症状的侵袭性肺炎球菌疾病的诱导因素之一<em&gt;肺炎链球菌</em&gt;运输。在这里，我们描述了一个混合感染的方法，使用婴儿小鼠调查这两个呼吸道病原体之间的协同作用。

利用生物发光成像之间调查的协同作用肺炎链球菌 A型流感病毒的婴儿小鼠

During the 1918 influenza virus pandemic, which killed approximately 50 million people worldwide, the majority of fatalities were not the result of ...

Research

JoVE Journal

Immunology and Infection

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

活细胞成像枯草芽孢杆菌肺炎链球菌使用自动定时显微镜

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肺炎链球菌在鼻内殖民期间炎症反应的特征

Nasopharyngeal colonization by Streptococcus pneumoniae is a prerequisite to invasion to the lungs or bloodstream1. This organism is capable of colonizing ...

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监测改变膜极性，膜完整性和细胞内离子浓度<em&gt;肺炎链球菌</em&gt;使用荧光染料

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There are over 90 different capsular serotypes of Streptococcus pneumoniae (the pneumococcus). As well as being a tool for understanding pneumococcal ...

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Latex agglutination reagents are widely used in microbial diagnosis, identification and serotyping. Streptococcus pneumoniae (the pneumococcus) is a major ...

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Influenza infection is associated with about 36,000 deaths and more than 200,000 hospitalizations every year in the United States. The continuous ...

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Neutrophils are the most abundant (40% to 75%) type of white blood cells and among the first inflammatory cells to migrate towards the site of ...

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流感病毒在小鼠感染模型中的研究

Influenza viruses cause over 500,000 deaths worldwide1 and are associated with an annual cost of 12 - 14 billion USD in the United States alone ...

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