Name: peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects
Uploaded: 2010-12-10T17:00:00
Duration: 7 min 53 s
Description: Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

National Institutes of Health, AR53579, Osteogenesis: Potentiëring met Bone Tropic Peptiden National Institutes of Health, AR056422, anabole werking van osteogeen peptiden National Institutes of Health, T32 AR050960, Skeletspierstelsel Tissue Repair and Regeneration DOD, PC051219 Nucleolin: A Novel Middelaar van prostaatkanker en Bone Marrow Endotheliale Celadhesie Ministerie van Defensie, PC061508, prostaatkanker Metastase Cell-Bone Marrow hechting Mediators

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De productie van deze video werd gesponsord door de New England Biolabs, Inc, die een aantal van de gebruikte reagentia in dit artikel oplevert.

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<td>Phage Display Peptide Library Kit-Ph.D-12</td>
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peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects

Twee nieuwe synthetische peptiden te versnellen botvorming en kan worden geleverd met behulp van een collageen matrix. Het doel van deze studie was om de effecten op het bot te repareren te onderzoeken in een unicortical defect model. Behandeling van mesenchymale cellen produceerde een stijging van de alkalische fosfatase-activiteit, toonde de vorming van knobbeltjes door de cellen, en verhoogde de expressie van genen voor runx2, osterix, bot sialoprotein, en osteocalcine. Een collageen sponsje nat met peptide bevorderde het herstel van botdefecten, terwijl de controlegroep was minder effectief. De resultaten van deze studie toonde aan dat mesenchymale cellen behandeld met peptide in vitro differentiëren naar osteogenesis, en dat de peptiden geleverd in vivo met behulp van een collageenspons de reparatie van unicortical gebreken te bevorderen.

Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Een faag display bibliotheek werd gebruikt om de peptide-sequenties die zich richten op het bot te identificeren. Het doel was om het effect van deze peptiden op mesenchymale cel differentiatie te onderzoeken en om het effect op het bot regeneratie te bepalen.

Peptiden van faag display bibliotheek Modulate genexpressie in mesenchymale cellen en versterken Osteogenesis in Unicortical Bone Gebreken

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects ...

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