Name: peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects
Uploaded: 2010-12-10T17:00:00
Duration: 7 min 53 s
Description: Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

건강, AR53579, Osteogenesis 국립 연구소 : 본 트로픽 펩티드와 Potentiation 보건 국립 연구소, AR056422, Osteogenic 펩티드의 근육 효과 보건 국립 연구소, T32 AR050960, Musculoskeletal 조직 정비 및 리제 너 레이션 국방부 PC051219 Nucleolin : 전립선암과 골수 내피 세포 접착의 소설 중재자 국방부, PC061508, 전립선 암 전이 셀 골수 접착 중재자

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이 비디오의 제작이 문서에서 사용되는 시약의 일부를 생산하고 뉴잉글랜드 Biolabs, Inc의 후원했다.

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<td>Phage Display Peptide Library Kit-Ph.D-12</td>
<td>&nbsp;</td>
<td>New England Biolabs</td>
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peptides from phage display library modulate gene expression in mesenchymal cells and potentiate osteogenesis in unicortical bone defects

두 소설 합성 펩티드는 뼈 형성을 촉진하고 콜라겐 매트릭스를 사용하여 전달할 수 있습니다. 본 연구의 목적은 unicortical 결함 모델에서 뼈 수리에 대한 효과를 조사하는 것이었다. mesenchymal 세포의 치료, 알칼리성 인산 가수 분해 효소의 활동 증가를 생산하는 세포에 의해 결절 형성을 보여주하고, runx2, osterix, 뼈 sialoprotein 및 osteocalcin에 대한 유전자의 표현을 증가했다. 컨트롤이 덜 효과​​적 반면 콜라겐 스폰지는 뼈 결함이 펩티드 홍보 복구로 적셔. 본 연구의 결과는 체외에서 펩타이드로 치료 mesenchymal 세포가 콜라겐 스폰지를 사용하여 생체내에 전달 펩티드가 unicortical 결함의 수리를 홍보, osteogenesis 대한 차별, 그리고 보여주었다.

Watch this Scientific Journal Video about Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects at JoVE.com

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

파지 디스플레이 라이브러리는 대상 뼈되는 펩타이드 시퀀스를 식별하는 데 사용되었다. 목표는 mesenchymal 세포 분화에서 이러한 펩티드의 효과를 조사하고 뼈 재생에 대한 그들의 영향을 결정하는 것이었다.

파지 디스플레이 도서관에서 펩티드는 Mesenchymal 세포의 유전자 발현을 조절하고 Unicortical 뼈 결함에 Osteogenesis을 힘을 주다

Two novel synthetic peptides accelerate bone formation and can be delivered using a collagen matrix. The aim of this study was to investigate the effects ...

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