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DOI :
10.3791/2366-v
•
9:02 min
March 5th, 2011
Chapters
0:05
Title
1:21
Basic Design of the Convective Thermocycler
2:48
Preparation and Loading of the PCR Reaction Mixture
3:42
Running the Reaction in the Convective Thermocycler
4:25
Performing Gel Electrophoresis Analysis
5:31
Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
8:33
Conclusion
我们描述了一种新的方法,通过聚合酶链反应(PCR)进行DNA复制。热对流是利用变性,退火,以及延长条件之间不断穿梭试剂保持反对在反应器的表面温度恒定。这在本质上简单设计的承诺,使快速PCR更方便。
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