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DOI :
10.3791/2366-v
•
9:02 min
March 5th, 2011
Chapters
0:05
Title
1:21
Basic Design of the Convective Thermocycler
2:48
Preparation and Loading of the PCR Reaction Mixture
3:42
Running the Reaction in the Convective Thermocycler
4:25
Performing Gel Electrophoresis Analysis
5:31
Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
8:33
Conclusion
我々は、ポリメラーゼ連鎖反応(PCR)を経由してDNA複製を実行する新規な方法を説明します。熱対流は、一定温度で反応器の対向面を維持することによって、変性アニーリング、および拡張子の条件の間に連続してシャトルの試薬に活かされている。この本質的にシンプルなデザインは、迅速なPCRをよりアクセシブルにすることを約束。
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