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DOI :
10.3791/2371-v
February 18th, 2011
Chapters
0:05
Title
1:24
Splenic B-Lymphocyte Isolation and Stimulation
5:42
Transfection of Producer Cells
7:26
Infection of Stimulated Splenic B cells
8:12
Flow Cytometry Analysis
8:40
Rescue of AID-Deficiency by Transduction of AID-Deficient B Cells with a Recombinant Retrovirus
8:57
Conclusion
一个有效率的制度中的一个基因的结构和功能分析体外脾B淋巴细胞的文化描述。这种方法需要利用一个辅助的自由,ecotrophic包装细胞系的重组逆转录病毒的生产。稳定,遗传兴趣在初级淋巴细胞基因的表达,是实现领导在接受类开关重组的B细胞表面抗体的生成。
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