Name: contrast enhanced vessel imaging using microct
Uploaded: 2011-01-27T17:30:00
Description: Watch this Scientific Journal Video about Contrast Enhanced Vessel Imaging using MicroCT at JoVE.com

<p class="jove_content">这项工作是支持部分由美国国立癌症研究所奖5R01CA133229 - 03。 </p>

<p class="jove_title"> 1。动物注射</p><p class="jove_content">静脉注射尾静脉注射前，尾部皮肤，应清洗与杀菌的目的异丙醇磨砂。然后应注射0.2 mL/20 g的20毫克碘/毫升的碘bloodpool造影剂（能天使160XL; Binito生物医学公司，ON，加拿大渥太华）通过使用低死腔静脉远端尾半消委会ü小鼠-100 28G半胰岛素注射器（＃329461产品，碧迪公司，新泽西州，美国）。 </p><p class="jove_title"> 2。动物的制备</p><p class="jove_content">注射10分钟后，动?...

<ol>
	<li>Holdsworth, D. W., Thornton, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-CT+in+small+animal+and+specimen+imaging.">Micro-CT in small animal and specimen imaging.</a> <em style='font-style: italic'>Trends in Biotechnology</em>. <b>20</b>, 417-424 (2002).</li><li>Kindlmann, G. L., Weinstein, D. M., Jones, G. M., Johnson, C. R., Capecchi, M. R., Keller, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Practical+Vessel+Imaging+by+Computed+Tomography+in+Live+Transgenic+Mouse+Models+for+Human+Tumors.">Practical Vessel Imaging by Computed Tomography in Live Transgenic Mouse Models for Human Tumors.</a> <em style='font-style: italic'>Molecular Imaging</em>. <b>4</b>, 417-424 (2005).</li></ol>

<p class="jove_content">这种增强血管成像使用microCT技术的首要目标是提供高质量数据集的分析在活体小鼠的血管网络生成的最佳方法。对于microCT更好的信号噪声比软组织是由较长的扫描时间实现意见和<sup>按次</sup> 2帧的数量增加数字成像，。 </p><p class="jove_content">船只识别严重依赖准确的软组织鉴定，并从骨分化后。能天使160XL是碘基于属性无线电高度不透明的财产和密度是不同形式的软组织和骨的。这使得它方便，...

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<td>eXIA 160XL</td>
<td>&nbsp;</td>
<td>Binito Biomedical, Inc.</td>
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contrast enhanced vessel imaging using microct

<p class="jove_content">显微计算机断层扫描（microCT）提供高分辨率容积成像的生活小动物的解剖结构。然而，本质上是不同的软组织和体液之间的对比差，微型<sup>CT</sup>图像1。在这种情况下，血管的可视化将成为一个几乎不可能完成的任务。为了克服这一外生造影剂可用于改善血管的可视化。在这里，我们提出了一个可视化在啮齿类动物模型的血管网的方法。通过使用一种长效的水性胶体多分散碘化血池造影剂，能天使160XL，我们发现在活的动物血管的图像采集参数和体积渲染技术优化。我们的研究结果表明，实现了出色的对比度血管的骨和软组织之间，多帧（至少5-8 /每帧视图），360-720意见（一个完整的360 °旋转）收购是强制性的。我们也展示了一个两维的传递函数的使用（体素的颜色和透明度是在CT值和梯度幅度按比例分配），在可视化的解剖和突出的利息，血管网络结构。这个有前途的工作奠定了使用转基因或异种荷瘤小鼠的抗血管生成的临床前研究的定性和定量评估的基础。 </p>

Watch this Scientific Journal Video about Contrast Enhanced Vessel Imaging using MicroCT at JoVE.com

Contrast Enhanced Vessel Imaging using MicroCT

<p class="jove_content">对比度增强小动物microCT血管成像是一种快速，成本效益和高吞吐量的串行技术<em&gt;原位</em&gt;检查肿瘤的发展，分析血管，滋养他们的网络，并为干预治疗的临床前研究（S）以下的肿瘤的反应。</p>

增强容器成像MicroCT

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between ...

Research

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