Name: heterotopic heart transplantation in mice
Uploaded: 2007-07-19T00:00:00
Description: Watch this Scientific Journal Video about Heterotopic Heart Transplantation in Mice at JoVE.com

捐助者的心脏的制备及收获： <ol><li style="text-align: left">该鼠标是与腹腔注射巴比妥和手术视野放在仰卧麻醉。 </li><li style="text-align: left">是一个长期的中线腹部切口。以暴露腹主动脉和下腔静脉，腹腔内容物用纱布缩回到左。 </li><li style="text-align: left"> 1毫升肝素（10u/ml）注入下腔静脉肝素。 </li><li style="text-align: left">开胸脊柱两侧的肋骨上存在分歧，前胸壁杠杆暴露心脏。 </li><li style="text-align: ...

<ol>
	<li>Corry, R. o. b. e. r. t. . J., Winn, H. e. n. r. y. . J., Russell, P. a. u. l. . S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Primarily+vascularized+allografts+of+heart+in+mice.">Primarily vascularized allografts of heart in mice.</a> Transplantation. 16, 343-350 (1973).</li></ol>

结果 我们已经成功地在小鼠异位心脏移植1000余例，并取得90％以上的成功率在不同品系小鼠，包括野生型，转基因和基因敲除小鼠。 讨论 由于博士。科里和罗素介绍，1973年在小鼠异位心脏移植模型，该模型已被证明是一个特别有价值的研究免疫排斥反应和开发新的免疫策略。这种模式可以更广泛地使用，因为现在出现了大量的转基因和基因敲除小鼠研究排斥/宽容的?...

heterotopic heart transplantation in mice

小鼠异位心脏移植已被广泛使用，因为它是由博士介绍。科里和罗素在1973年。它特别适用于研究排斥反应和免疫反应，现在，新的转基因和基因敲除小鼠可宝贵的，已经开发和大量的免疫试剂。心脏移植模型是严格小于皮肤移植模型，虽然在技术上更具挑战性。我们已经开发出一种改性技术，并已完成超过1000对小鼠异位心脏移植成功的病例。升主动脉和腹主动脉吻合时，两呆缝线放置捐助小号升主动脉与受体腹主动脉的近端和远端的顶点，然后使用主动脉两侧11-0继续缝线缝合吻合。留缝线吻合更容易和11-0是一个理想的缝合的大小，以避免出血和血栓形成。 肺动脉和下腔静脉吻合时，两呆缝线捐助小号肺动脉近端顶点与收件人小号下腔静脉远端心尖。下腔静脉和捐助小号肺动脉左侧壁与下腔静脉内继续缝合封闭后，与近端顶点住宿结缝合下腔静脉的右侧壁和捐助小号肺动脉外下腔洞穴，用10-0缝线缝合继续封闭。这种方法更容易执行，因为只是一方的下腔静脉和10-0缝线吻合，是正确的大小，以避免出血和血栓形成。在这篇文章中，我们提供的技术，以补充视频的细节。

Watch this Scientific Journal Video about Heterotopic Heart Transplantation in Mice at JoVE.com

Heterotopic Heart Transplantation in Mice

小鼠异位心脏移植模型已被证明许多研究者排斥反应和免疫反应机制研究的重要方法。然而，所涉及的技术仍然充满挑战。通过修改标准的技术，我们已与超过1000移植成功。

小鼠异位心脏移植

The mouse heterotopic heart transplantation has been used widely since it was introduced by Drs. Corry and Russell in 1973. It is particularly valuable ...

Research

JoVE Journal

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