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DOI :
10.3791/2381-v
January 14th, 2011
Chapters
0:05
Title
0:55
Introduction
1:48
Mouse Model of Breast Cancer
2:14
Isolation of Lymphocytes
3:44
Determine Fold Expansion of T Cells by Cell Counts and Flow Cytometry Analysis
4:58
Determine Tumor-Specificity of the ex vivo Expanded T Cells
5:47
Selective Expansion of Tumor-Reactive T Cells with Effector Anti-Tumor Function
6:53
Conclusion
为有效的协议体外描述从肿瘤引流淋巴结或其他淋巴组织的继发性肿瘤轴承主机的肿瘤反应性T细胞的扩张。该协议有选择地扩大肿瘤特异性T细胞过继免疫治疗乳腺癌。
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