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DOI :
10.3791/2390-v
October 19th, 2010
Chapters
0:00
Title
1:43
tRNA Preparation
5:28
Expression and Purification of the Recombinant E. histolytica Dnmt2 (Ehmeth) Protein in E. coli BL21
8:06
in vitro tRNA Methylation Assay
10:30
Representative Results
12:02
Conclusion
这个协议描述为合成tRNA的衬底准备阿米巴的tRNA / DNA的甲基转移酶2(Dnmt2)的同源性Ehmeth和甲基转移酶活性的措施。这种实验方法可用于调查其他Dnmt2蛋白的活性。
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