October 19th, 2010
•This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.
Tags
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved