Name: mechanical stimulation of stem cells using cyclic uniaxial strain
Uploaded: 2007-07-29T00:00:00
Description: Watch this Scientific Journal Video about Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain at JoVE.com

0天 - 灭菌前一天的实验<ol><li>材料放入塑料桶和消毒，用70％酒精2小时： <ul><li>钱伯斯</li><li>盖</li><li>相框（3件） </li><li>螺丝</li><li>橡胶垫片</li><li>镊子</li><li>剪刀</li><li>六角扳手</li></ul></li><li>用Aquet肥皂和蒸馏水清洁膜。 </li><li>超声在10分钟的70％的酒精膜。 </li><li>成塑料平方米美食广场的清洁膜。如果使用图案膜，确保图案的一面是面对（到膜的一侧喷酒精和观看的液体运行的凹槽）。 <...

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	<li>Banes, A. J., Gilbert, J., Taylor, D., Monbureau, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+vacuum-operated+stress-providing+instrument+that+applies+static+or+variable+duration+cyclic+tension+or+compression+to+cells+in+vitro.">A new vacuum-operated stress-providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro.</a> J Cell Sci. 75, 35-42 (1985).</li><li>Park, J. S., Chu, J. S., Cheng, C., Chen, F., Chen, D., Li, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+effects+of+equiaxial+and+uniaxial+strain+on+mesenchymal+stem+cells.">Differential effects of equiaxial and uniaxial strain on mesenchymal stem cells.</a> Biotechnol. Bioeng. 88, 359-368 (2004).</li><li>Kurpinski, K., Chu, J., Hashi, C., Li, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anisotropic+mechanosensing+by+mesenchymal+stem+cells.">Anisotropic mechanosensing by mesenchymal stem cells.</a> Proc. Natl. Acad. Sci. U S A. 103, 16095-16100 (2006).</li></ol>

巴内斯等。首次报道在体外培养细胞的机械性刺激，通过使用一个灵活的弹性基材提供机械力1细胞的系统使用。这段时间以来，这种设计上的许多变化已经构思和利用。几个机械牵张系统市售的名义下“Flexercell”（Flexcell国际公司），而一些实验室使用定制的设备。在这个视频协议，我们已经描述了一个这样的设备的安装和使用，在我们的实验室。 本议定书中所描述的定制的“拉伸机”已被用来?...

媒体上的注意事项：这些单轴拉伸实验是容易发生污染的生物反应器设置的复杂性。为了解决这个问题，我们已经尝试了各种组合的抗生素和抗真菌剂。 1％fungizone，通常是必要的，随着抗生素的组合。要么使用钢笔/链球菌结合，或用卡那霉素和庆大霉素的组合（但不要尝试使用组合/鸡尾酒三个或更多的抗生素，因为它可能导致抗药性突变的细菌，也可能是有害的细胞） 。

mechanical stimulation of stem cells using cyclic uniaxial strain

在生物组织的发展和维护的机械力的作用是有据可查的，包括几个机械监管的现象，如骨重塑，肌肉肥厚，平滑肌细胞的可塑性。然而，涉及的力量往往是非常复杂和困难的监视和控制体内。为了更好地研究细胞的机械力的影响，我们已经制定了在体外采用单向循环拉伸应变的弹性膜贴壁细胞培养的方法。这种方法采用了机动凸轮转子系统定制设计的生物反应器，以申请所需的力。在这里，我们现在一步一步的视频协议，演示了如何组装的每一个“舒展室”的各个组成部分，在这种情况下，包括micropatterned地形的方向与应变方向的细胞与硅膜。我们还描述了消毒商会，播种到膜细胞，到生物反应器的闭锁室，和调整的力学参数（即幅度和应变率）的程序。在这个特殊的协议中所述的程序，具体到与10微米宽的通道方向平行方向的应变硅膜播种人类间质干细胞。然而，在这个系统中提出的方法和材料是灵活的，足以容纳一个关于这一主题的变化：应变速率，幅度，持续时间，细胞类型，地形膜，膜涂层，等等都可以根据所需的应用程序或的结果。这是一个可靠的方法，调查体外细胞应用于单轴拉伸应变的影响。

Watch this Scientific Journal Video about Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain at JoVE.com

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

它被广泛了解，在体内的机械力可以影响细胞分化和增殖。在这里，我们提出了一个视频协议，显示了使用一个定制的生物反应器提供单向循环拉伸应变干细胞培养灵活micropatterned基板。

采用循环单轴应变的干细胞的机械性刺激

The role of mechanical forces in the development and maintenance of biological tissues is well documented, including several mechanically regulated ...

Research

JoVE Journal

Biology

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

从小鼠胚胎干细胞的造血干细胞的推导

A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny.  Embryonic stem cells (ESC) are ...

科研

生物学

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

图案的使用生物倒装芯片的胚胎干细胞

Cell-cell interactions consisting of diffusible signaling and cell-cell contact (juxtacrine signaling) are important in numerous biological processes such ...

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

机械性刺激，集成光学和原子力显微镜研究活细胞的反应

To understand the mechanism by which living cells sense mechanical forces, and how they respond and adapt to their environment, a new technology able to ...

Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

使用胚体冰冻切片分析的多能干细胞

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocyst-stage early mammalian embryos 1. A crucial stage in the ...

Quantifying the Frequency of Tumor-propagating Cells Using Limiting Dilution Cell Transplantation in Syngeneic Zebrafish

量化传播肿瘤细胞的频率使用斑马鱼的同源有限稀释细胞移植

Self-renewing cancer cells are the only cell types within a tumor that have an unlimited ability to promote tumor growth, and are thus known as ...

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

微型机械的肺组织利用原子力显微镜表征

Matrix stiffness strongly influences growth, differentiation and function of adherent cells1-3.  On the macro scale the stiffness of tissues and organs ...

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

生物物理检测探头相间细胞核的力学性能：基材应变的应用和微针操纵

In most eukaryotic cells, the nucleus is the largest organelle and is typically 2 to 10 times stiffer than the surrounding cytoskeleton; consequently, the ...

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

人类体细胞重编程为诱导多能干细胞（iPS细胞）用绿色荧光蛋白逆转录病毒载体

Human embryonic stem cells (hESCs) are pluripotent and an invaluable cellular sources for in vitro disease modeling and regenerative medicine1. It has ...

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

机械性刺激软骨细胞琼脂糖凝胶

Articular cartilage suffers from a limited repair capacity when damaged by mechanical insult or degraded by disease, such as osteoarthritis. To remedy ...

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

机械刺激引起的钙波传播的细胞单层：牛角膜内皮细胞的例子

Intercellular communication is essential for the coordination of physiological processes between cells in a variety of organs and tissues, including the ...