Name: isolation of genomic dna from mouse tails
Uploaded: 2007-07-29T00:00:00
Description: Watch this Scientific Journal Video about Isolation of Genomic DNA from Mouse Tails at JoVE.com

<ol style="margin-top: 0in"><li> Knip de staart stukken (3mm) en Mark oren. </li><li> Voeg 720 <span style="font-family: Symbol;">m</span> l STE en 30 <span style="font-family: Symbol;">m</span> l proteïnase K (10 mg / ml bouillon). </li><li> Incubeer bij <span><span style="font-family: Symbol;">55</span></span> ° C bij verhitting blok en vortex elk uur gedurende 3 uur bij topsnelheid voor 10 seconden. </li><li> Inactiveren proteïnase K bij <span><span style="font-family: Symbol;">70</span></span> ° C gedurende 5 minuten. </li><li> Quench o...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>STE</td>
<td>Buffer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>100 mm Tris; pH 8.5 /
5 mM EDTA /
0.2% SDS /
200 mM NaCl /
 in H2O
</td>
</tr>
<tr>
<td>TE</td>
<td>Buffer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>100 mM Tris; pH 5.0 /
1 mM EDTA /
10 mM NaCl
</td>
</tr>		</tbody>
		</table>
		</div>
			<div>
					</div>
		

isolation of genomic dna from mouse tails

Watch this Scientific Journal Video about Isolation of Genomic DNA from Mouse Tails at JoVE.com

Isolation of Genomic DNA from Mouse Tails

Isolatie van Genomic DNA van muis Tails

Research

JoVE Journal

Biology

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Extractie met een hoog molecuulgewicht Genomic DNA uit bodems en sedimenten

The soil microbiome is a vast and relatively unexplored reservoir of genomic diversity and metabolic innovation that is intimately associated with ...

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Genereren van RNA / DNA-Hybriden in Genomic DNA door omzetting door middel RNA-bevattende Oligonucleotiden

Synthetic short nucleic acid polymers, oligonucleotides (oligos), are the most functional and widespread tools of molecular biology. Oligos can be ...

Isolation of Retinal Stem Cells from the Mouse Eye

Isolatie van het netvlies stamcellen van de muis Eye

The adult mouse retinal stem cell (RSC) is a rare quiescent cell found within the ciliary epithelium (CE) of the mammalian eye1,2,3. The CE is made up of ...

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Genomische MRI - een openbare hulpbron voor het bestuderen van Sequence patronen binnen Genomic DNA

Non-coding genomic regions in complex eukaryotes, including intergenic areas, introns, and untranslated segments of exons, are profoundly non-random in ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generatie van Muizen Afgeleid van geïnduceerde pluripotente stamcellen

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Een praktische en nieuwe methode om Genomic DNA Uittreksel uit Bloedafname Kits voor Plasma Protein Preservation

Laboratory tests can be done on the cellular or  fluid portions of the blood. The use of different blood collection tubes  determines the portion of the ...

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Selectieve vangen van 5-hydroxymethylcytosine van genomisch DNA

5-methylcytosine (5-mC) constitutes ~2-8% of the total cytosines in human genomic DNA and impacts a broad range of biological functions, including gene ...

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Isolatie van Primaire Myofibroblasten van Mouse and Human Colon Tissue

The myofibroblast is  a stromal cell of the gastrointestinal (GI) tract that has been gaining  considerable attention for its critical role in many GI ...

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

Isolatie van microvasculaire endotheelcellen Buizen van Mouse Resistance Arteries

The control of blood flow by the resistance vasculature regulates the supply of oxygen and nutrients concomitant with the removal of metabolic ...

Isolation of Myofibroblasts from Mouse and Human Esophagus

Isolatie van Myofibroblasten van muis en mens slokdarm

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, ...