Name: isolation of genomic dna from mouse tails
Uploaded: 2007-07-29T00:00:00
Description: Watch this Scientific Journal Video about Isolation of Genomic DNA from Mouse Tails at JoVE.com

<ol style="margin-top: 0in"><li> Kuyruk parçaları (3mm) ve işareti kulakları kesin. </li><li> 720 <span style="font-family: Symbol;">m</span> l STE ve 30 <span style="font-family: Symbol;">m</span> l Proteinaz K (10 mg / ml stok) ekleyin. </li><li> 55 inkübe <span><span style="font-family: Symbol;">°</span></span> C ısıtma bloğu ve vorteks her saat 3 saat için 10 saniye boyunca en yüksek hızda. </li><li> 70 <span><span style="font-family: Symbol;">°</span></span> C&#39;de 5 dakika proteinaz K inaktive. </li><li> 5 dakika boyunca bu...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>STE</td>
<td>Buffer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>100 mm Tris; pH 8.5 /
5 mM EDTA /
0.2% SDS /
200 mM NaCl /
 in H2O
</td>
</tr>
<tr>
<td>TE</td>
<td>Buffer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>100 mM Tris; pH 5.0 /
1 mM EDTA /
10 mM NaCl
</td>
</tr>		</tbody>
		</table>
		</div>
			<div>
					</div>
		

isolation of genomic dna from mouse tails

Watch this Scientific Journal Video about Isolation of Genomic DNA from Mouse Tails at JoVE.com

Isolation of Genomic DNA from Mouse Tails

Fare Tails Genomik DNA izolasyonu

Research

JoVE Journal

Biology

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Topraklar ve tortulları Yüksek Moleküler Ağırlık Genomik DNA çıkarımı

The soil microbiome is a vast and relatively unexplored reservoir of genomic diversity and metabolic innovation that is intimately associated with ...

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

RNA içeren Oligonükleotidler kullanarak Dönüşüm Genomik DNA, RNA / DNA Hibritlerin Üretimi

Synthetic short nucleic acid polymers, oligonucleotides (oligos), are the most functional and widespread tools of molecular biology. Oligos can be ...

Isolation of Retinal Stem Cells from the Mouse Eye

Fare Göz Retina Kök Hücre İzolasyonu

The adult mouse retinal stem cell (RSC) is a rare quiescent cell found within the ciliary epithelium (CE) of the mammalian eye1,2,3. The CE is made up of ...

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

MR Genomik - Sıra Desenler Genomik DNA içinde incelenmesi için Kamu Kaynak

Non-coding genomic regions in complex eukaryotes, including intergenic areas, introns, and untranslated segments of exons, are profoundly non-random in ...

Generation of Mice Derived from Induced Pluripotent Stem Cells

İsteyerek Pluripotent Kök Hücrelerinin Elde Edilen Fare Üretimi

The production of induced pluripotent stem cells (iPSCs) from somatic cells provides a means to create valuable tools for basic research and may also ...

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Plazma Protein Koruma Kan Alma Kitleri Genomik DNA Ayıklama Pratik ve Yeni Bir Yöntem

Laboratory tests can be done on the cellular or  fluid portions of the blood. The use of different blood collection tubes  determines the portion of the ...

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

Genomik DNA 5-hydroxymethylcytosine seçici Yakalama

5-methylcytosine (5-mC) constitutes ~2-8% of the total cytosines in human genomic DNA and impacts a broad range of biological functions, including gene ...

Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue

Fare ve İnsan Kolon Doku Primer miyofibroblastlar İzolasyonu

The myofibroblast is  a stromal cell of the gastrointestinal (GI) tract that has been gaining  considerable attention for its critical role in many GI ...

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries

Fare Direnç arterlerden mikrovasküler endotel Tüpler İzolasyonu

The control of blood flow by the resistance vasculature regulates the supply of oxygen and nutrients concomitant with the removal of metabolic ...

Isolation of Myofibroblasts from Mouse and Human Esophagus

Fare ve İnsan Özofagus gelen miyofibroblastların izolasyonu

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, ...