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DOI :
10.3791/2461-v
February 1st, 2011
Chapters
0:05
Title
3:39
Measuring the Growth of E. coli Clones.
6:35
Addition of the Assay Mix to Each Plate
7:47
Reading Absorbance of Assayed Clones
1:05
Replication of the Metagenomic Library Plates
9:17
Representative Results of a Solution-Based Screen for a Cellulose Activity from Metagenomic Libraries
9:56
Conclusion
このプロトコルは、大腸菌で発現メタゲノムライブラリーからのセルロース分解活性のためのハイスループットスクリーニングを説明します。画面には、ソリューションベースと高度に自動化され、吸光度測定など最終的な読み出しと384ウェルマイクロプレートでのワンポット化学を使用しています。
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