Name: measuring circadian and acute light responses in mice using wheel running activity
Uploaded: 2011-02-04T18:00:00
Description: Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

这项工作是由美国国立卫生研究院授予R01 GM76430，大卫和露西尔帕卡德基金会和艾尔弗雷德斯​​隆基金会的资助。

1。设备安装<ol><li>设立任何行为实验时，动物群体的制备是非常重要的的。车轮运行的活动，所有的小鼠需要为男性，年龄匹配的，并且如果可能的话从兄弟姐妹交配。理想的情况是年轻的老鼠，大约3个月大，用于车轮运行的活动。 </li><li>将车轮上的任何动物之前，房间需要完全安装。这包括准备1轮，1轮革命探头，在每个笼和充足的食物和水2周的底部少量的鼠标床上用品每笼。 </li><li>房间?...

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昼夜节律进行了测量，并记录在整个历史上的各种生物。虽然我们有专门描述记录小鼠的活动节律的方法，这种技术可以很容易地修改，以衡量在其他啮齿类动物，如仓鼠和大鼠，这往往是昼夜研究的节奏。然而，freerunning期间和时间重新夹带其他生物体会有所不同。例如，在不断黑暗仓鼠freerunning期间为24.0小时，而在小鼠，这是不到24个小时。在这种情况下，你是一个有机体，这是不适合车轮运...

The article <a href="http://www.jove.com/video/2463/">Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity</a>&#160;has been retracted by the journal due to concerns regarding the validity of the presented data.

The article Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity has been retracted by the journal due to concerns regarding the validity of the presented data.

Retraction: Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

<ul><li>小型发射机11.5厘米的车轮<a href="http://minimitter.respironics.com/home/">http://minimitter.respironics.com/home/</a> </li><li>微型发射机进行数据采集重要的查看<a href="http://minimitter.respironics.com/home/">软件</a> http://minimitter.respironics.com/home/ </li><li> Actimetrics时钟进行数据分析实验室<a href="http://www.actimetrics.com/ClockLab/">http://www.actimetrics.com/ClockLab/</a> </li></ul>

measuring circadian and acute light responses in mice using wheel running activity

昼夜节律的生理功能，周期多期（约24小时昼夜大约：近似的日均：天）1，2。他们是负责定时睡眠/唤醒周期和激素分泌。由于这个时机不正是24小时，它是太阳日同步光输入。这是通过光的输入从视网膜视交叉上核（SCN）在脑和周围组织的环境光线暗周期3-7其他地区同步外设时钟作为主起搏器。这种环境的光线暗周期的节奏对齐举办特殊的生理活动，以正确的时间生态位，这是生存的关键8。例如，老鼠白天睡觉，都在夜间活动。这种能力，巩固活动，无论是一天的浅色或深色的部分被称为昼夜photoentrainment和要求光输入生物钟 9 。小鼠在夜间活动，是强大的，尤其是在一个跑轮。测量这种行为是一种微创的方法，可用于评估的昼夜系统的功能以及光输入到这个系统。将这里介绍的方法用于研究生物钟，光输入到这个系统，以及光轮的运行行为的直接影响。

Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

本文将回顾的方法可以用来确定昼夜在小鼠体内的功能和光响应。

测量小鼠使用车轮运行活动的昼夜和急性对光反应

Circadian rhythms are physiological functions that cycle over a period of approximately 24 hours (circadian- circa: approximate and diem: day)1, 2.  They ...

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含量自发活动，研究昼夜节律和睡眠参数果蝇</em

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总RNA的分离和下游下一代测序和基因表达谱的非激光捕获显微切割离散小鼠脑地区显微镜方法

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运行轮活动在啮齿类动物的昼夜节律的记录和分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

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光偏好的检测，以研究的先天和昼夜调节感光特性<em&gt;果蝇</em&gt;幼虫

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

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The retina is the gateway to the visual system. To understand visual signal processing mechanisms, we investigate retinal neural network functions. ...

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印刷业记录程序在小鼠体内测量睡眠

Recording of the epidural electroencephalogram (EEG) and electromyogram (EMG) in small animals, like mice and rats, has been pivotal to study the ...

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