Name: measuring circadian and acute light responses in mice using wheel running activity
Uploaded: 2011-02-04T18:00:00
Description: Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

This work was funded by the NIH grant R01 GM76430, David and Lucille Packard Foundation, and the Alfred Sloan Foundation.

1. Equipment Setup
<ol>
 <li>Preparation of animal groups is very important when setting up any behavioral experiment. For wheel running activity, all mice need to be male, age matched, and if possible from sibling matings. Ideally younger mice, approximately 3 months old, are used for wheel running activity.</li>
 <li> Prior to placing any animals on wheels, the room needs to be completely setup. This includes preparing each cage with 1 wheel, 1 wheel revolution probe, a small amount of mouse bedding in the b...

<ol>
	<li>Aschoff, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circadian+timing.">Circadian timing.</a> Ann N Y Acad Sci. 423, 442-468 (1984).</li><li>Pittendrigh, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Temporal+organization:+reflections+of+a+Darwinian+clock-watcher.">Temporal organization: reflections of a Darwinian clock-watcher.</a> Annu Rev Physiol. 55, 16-54 (1993).</li><li>Abrahamson, E. E., Moore, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suprachiasmatic+nucleus+in+the+mouse:+retinal+innervation,+intrinsic+organization+and+efferent+projections.">Suprachiasmatic nucleus in the mouse: retinal innervation, intrinsic organization and efferent projections.</a> Brain Res. 916, 172-191 (2001).</li><li>Guler, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melanopsin+cells+are+the+principal+conduits+for+rod-cone+input+to+non-image-forming+vision.">Melanopsin cells are the principal conduits for rod-cone input to non-image-forming vision.</a> Nature. 453, 102-105 (2008).</li><li>Hatori, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inducible+ablation+of+melanopsin-expressing+retinal+ganglion+cells+reveals+their+central+role+in+non-image+forming+visual+responses.">Inducible ablation of melanopsin-expressing retinal ganglion cells reveals their central role in non-image forming visual responses.</a> PLoS One. 3, e2451-e2451 (2008).</li><li>Nelson, R. J., Zucker, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Photoperiodic+control+of+reproduction+in+olfactory-bulbectomized+rats.">Photoperiodic control of reproduction in olfactory-bulbectomized rats.</a> Neuroendocrinology. 32, 266-271 (1981).</li><li>Reppert, S. M., Weaver, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Coordination+of+circadian+timing+in+mammals.">Coordination of circadian timing in mammals.</a> Nature. 418, 935-941 (2002).</li><li>Ouyang, Y., Andersson, C. R., Kondo, T., Golden, S. S., Johnson, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resonating+circadian+clocks+enhance+fitness+in+cyanobacteria.">Resonating circadian clocks enhance fitness in cyanobacteria.</a> Proc Natl Acad Sci U S A. 95, 8660-8664 (1998).</li><li>Hattar, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melanopsin+and+rod-cone+photoreceptive+systems+account+for+all+major+accessory+visual+functions+in+mice.">Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice.</a> Nature. 424, 76-81 (2003).</li><li>Legates, T. A., Dunn, D., Weber, E. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accelerated+re-entrainment+to+advanced+light+cycles+in+BALB/cJ+mice.">Accelerated re-entrainment to advanced light cycles in BALB/cJ mice.</a> Physiol Behav. 98, 427-432 (2009).</li><li>Minors, D. S., Waterhouse, J. M., Wirz-Justice, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+human+phase-response+curve+to+light.">A human phase-response curve to light.</a> Neurosci Lett. 133, 36-40 (1991).</li><li>Summer, T. L., Ferraro, J. S., McCormack, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phase-response+and+Aschoff+illuminance+curves+for+locomotor+activity+rhythm+of+the+rat.">Phase-response and Aschoff illuminance curves for locomotor activity rhythm of the rat.</a> Am J Physiol. 246, 299-304 (1984).</li><li>Redlin, U., Mrosovsky, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Masking+of+locomotor+activity+in+hamsters.">Masking of locomotor activity in hamsters.</a> J Comp Physiol A. 184, 429-437 (1999).</li><li>Aschoff, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exogenous+and+endogenous+components+in+circadian+rhythms.">Exogenous and endogenous components in circadian rhythms.</a> Cold Spring Harb Symp Quant Biol. 25, 11-28 (1960).</li><li>Refinetti, R., Menaker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+circadian+rhythm+of+body+temperature.">The circadian rhythm of body temperature.</a> Physiol Behav. 51, 613-637 (1992).</li><li>Scheer, F. A., Czeisler, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Melatonin,+sleep,+and+circadian+rhythms.">Melatonin, sleep, and circadian rhythms.</a> Sleep Med Rev. 9, 5-9 (2005).</li><li>Dudley, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altered+patterns+of+sleep+and+behavioral+adaptability+in+NPAS2-deficient+mice.">Altered patterns of sleep and behavioral adaptability in NPAS2-deficient mice.</a> Science. 301, 379-3783 (2003).</li></ol>

Circadian rhythms have been measured and recorded in various organisms throughout history. While we have specifically described the method for recording activity rhythms in mice, this technique can be easily modified to measure rhythms in other rodents such as hamsters and rats, which are often used in circadian studies. However, the freerunning period and time to re-entrain in other organisms will vary. For instance, the freerunning period of a hamster in constant darkness is 24.0 hours, while in mice it is less than 24...

The article <a href="http://www.jove.com/video/2463/">Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity</a>&#160;has been retracted by the journal due to concerns regarding the validity of the presented data.

The article Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity has been retracted by the journal due to concerns regarding the validity of the presented data.

Retraction: Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

<ul>
	<li>Mini Mitter 11.5 cm running wheels <a href="http://minimitter.respironics.com/home/">http://minimitter.respironics.com/home/</a></li>
	<li>Mini Mitter Vital View Software for Data Acquisition <a href="http://minimitter.respironics.com/home/">http://minimitter.respironics.com/home/</a></li>
	<li>Actimetrics Clock lab for data analysis <a href="http://www.actimetrics.com/ClockLab/">http://www.actimetrics.com/ClockLab/</a></li>
</ul>

measuring circadian and acute light responses in mice using wheel running activity

Circadian rhythms are physiological functions that cycle over a period of approximately 24 hours (circadian- circa: approximate and diem: day)1, 2. They are responsible for timing our sleep/wake cycles and hormone secretion. Since this timing is not precisely 24-hours, it is synchronized to the solar day by light input. This is accomplished via photic input from the retina to the suprachiasmatic nucleus (SCN) which serves as the master pacemaker synchronizing peripheral clocks in other regions of the brain and peripheral tissues to the environmental light dark cycle3-7. The alignment of rhythms to this environmental light dark cycle organizes particular physiological events to the correct temporal niche, which is crucial for survival8. For example, mice sleep during the day and are active at night. This ability to consolidate activity to either the light or dark portion of the day is referred to as circadian photoentrainment and requires light input to the circadian clock9. Activity of mice at night is robust particularly in the presence of a running wheel. Measuring this behavior is a minimally invasive method that can be used to evaluate the functionality of the circadian system as well as light input to this system. Methods that will covered here are used to examine the circadian clock, light input to this system, as well as the direct influence of light on wheel running behavior.

Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

This article will review methods that can be used to determine circadian function and light responsiveness in mice.

Circadian rhythms are physiological functions that cycle over a period of approximately 24 hours (circadian- circa: approximate and diem: day)1, 2.  They ...

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