Name: measuring circadian and acute light responses in mice using wheel running activity
Uploaded: 2011-02-04T18:00:00
Description: Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

この作品は、NIHの助成R01 GM76430、ダビデとルシールパッカード財団、およびアルフレッドスローン財団によって資金を供給された。

1。機器のセットアップ<ol><li>どんな行動実験をセットアップするときに動物のグループの準備は非常に重要です。ホイール実行中のアクティビティのために、すべてのマウスが一致した男性、年齢にする必要があります、と兄妹交配から、可能であれば。約3ヶ月理想的には若いマウスは、ホイール実行中のアクティビティに使用されます。 </li><li>車輪の上の任意の動物を配置する前に、?...

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概日リズムは歴史を通して様々な生物で測定し、記録されています。我々は、特にマウスでの活動リズムを記録するための方法を説明しているが、この手法は簡単にそのような多くの場合、概日の研究で使用されているハムスターやラットなど他のげっ歯類でリズムを測定するように変更することができます。しかし、他の生物の再同調にfreerunning期間と時間が異なります。マウスではそれ?...

The article <a href="http://www.jove.com/video/2463/">Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity</a>&#160;has been retracted by the journal due to concerns regarding the validity of the presented data.

The article Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity has been retracted by the journal due to concerns regarding the validity of the presented data.

Retraction: Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

<ul><li>車輪実行ミニミッター11.5センチメートル<a href="http://minimitter.respironics.com/home/">http://minimitter.respironics.com/home/を</a> </li><li>データ収集のためのミニミッターバイタルビューソフトウェア<a href="http://minimitter.respironics.com/home/">http://minimitter.respironics.com/home/</a> </li><li>データ分析のためのActimetricsクロックラボ<a href="http://www.actimetrics.com/ClockLab/">http://www.actimetrics.com/ClockLab/</a> </li></ul>

measuring circadian and acute light responses in mice using wheel running activity

1、2概日リズムは約24時間の期間（：：近似および日当日概-年頃）を介して生理機能、そのサイクルです。彼らは私たちの睡眠/覚醒サイクルとホルモンの分泌のタイミングを取る責任があります。このタイミングは正確に24時間ではないので、それは光入力で太陽の日に同期されます。これは、網膜からの環境光の明暗周期3-7の脳や末梢組織の他の地域で、周辺クロックを同期させるマスターのペースメーカーとして機能する視交叉上核（SCN）への光の入力によって行われます。この環境光暗サイクルのリズムのアラインメントは、生存8の不可欠な正しい時間的ニッチ、に特定の生理学的イベントを開催。例えば、マウスは、日中寝ると夜にアクティブになります。日の光や暗い部分のいずれかに活動を統合するこの機能は、概日photoentrainmentと呼ばれ、概日時計9に光入力が必要です。夜にマウスの活動は、特にホイールの回転の存在下で堅牢です。この動作を測定することは、概日システムの機能性だけでなく、このシステムへの光入力を評価するために使用できる低侵襲な方法です。ここにカバーされるメソッドは、概日時計、このシステムに入力される光だけでなく、車輪実行動作上の光の直接の影響を調べるために使用されます。

Read this Scientific Article Protocol about Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity at JoVE.com

Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity

この記事では、マウスの概日機能と光の応答を判断するために使用できる方法を検討します。

ホイール実行中のアクティビティを使用して、マウスの概日リズムと急性ライト応答を測定する

Circadian rhythms are physiological functions that cycle over a period of approximately 24 hours (circadian- circa: approximate and diem: day)1, 2.  They ...

Research

JoVE Journal

Neuroscience

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila

に概日リズムと睡眠のパラメータを研究するために運動活性を測定するショウジョウバエ</em

Most life forms exhibit daily rhythms in cellular, physiological and behavioral phenomena that are driven by endogenous circadian (&equiv;24 hr) ...

Measuring Motor Coordination in Mice

マウスにおける運動協調性の測定

Mice are increasingly being used in behavioral neuroscience, largely replacing rats as the behaviorist's animal of choice. Before aspects of behavior such ...

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling  |

トータルRNAの単離およびダウンストリーム次世代シーケンシングと遺伝子発現プロファイリングのための離散マウスの脳領域のマイクロダイセクションのための非レーザーキャプチャー顕微鏡のアプローチ

As technological platforms, approaches such as next-generation sequencing, microarray, and qRT-PCR have great promise for expanding our understanding of ...

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

急性海馬スライスにおけるシナプス誘発アストログリアとニューロン活動の二重の電気生理学的記録

Astrocytes form together with neurons tripartite synapses, where they integrate and modulate neuronal activity. Indeed, astrocytes sense neuronal inputs ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

げっ歯類におけるランニングホイール活動の概日リズムの記録および分析

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

Light Preference Assay to Study Innate and Circadian Regulated Photobehavior in Drosophila Larvae

に自然免疫とサーカディアン規制Photobehaviorを勉強する光プアッセイ<em&gt;ショウジョウバエ</em&gt;幼虫

Light acts as environmental signal to control animal behavior at various levels. The Drosophila larval nervous system is used as a unique model to answer ...

Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

パッチクランプ技術を用いたダーク適応、マウス網膜スライス標本におけるニューロンにおける記録光誘発シナプス後応答

The retina is the gateway to the visual system. To understand visual signal processing mechanisms, we investigate retinal neural network functions. ...

Polygraphic Recording Procedure for Measuring Sleep in Mice

マウスで睡眠を測定するためのポリグラフ記録手順

Recording of the epidural electroencephalogram (EEG) and electromyogram (EMG) in small animals, like mice and rats, has been pivotal to study the ...

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo

の記録細胞内の電圧応答のための電気生理学的手法<em&gt;ショウジョウバエ</em&gt;光刺激に感光体と介在ニューロン<em&gt;インビボ</em

Voltage responses of insect photoreceptors and visual interneurons can be accurately recorded with conventional sharp microelectrodes. The method ...

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Thy1を用いた一次体性感覚野における神経活動のイメージング-GCaMP6s トランスジェニック マウス

The mammalian brain exhibits marked symmetry across the sagittal plane. However, detailed description of neural dynamics in symmetric brain regions in ...