Name: acute myocardial infarction in rats
Uploaded: 2011-02-16T17:00:00
Description: Watch this Scientific Journal Video about Acute Myocardial Infarction in Rats at JoVE.com

BKM поддерживается Национальным институтом здоровья (NIH) / Национальная легкого сердца и крови институт (NHLBI) грант (R01-HL085487). MH поддерживается Американской ассоциации сердца Национальный (AHA) Ученый грантов на цели развития (ГРС) и Общества гериатрической кардиологии.

1. Подготовка рабочей зоны <ol><li> Рабочей поверхности сначала подготовленную дезинфекции области с 70% этанола. </li><li> Все хирургические инструменты должны стерилизоваться горячей бусинка стерилизатор до операции (а в отдельных операций между крысой). Эти инструменты включают в себя: хир...

<ol>
	<li>Hunt, S. A., Baker, D. W., Chin, M. H., Cinquegrani, M. P., Feldmanmd, A. M., Francis, G. S., Ganiats, T. G., Goldstein, S., Gregoratos, G., Jessup, M. L., Noble, R. J., Packer, M., Silver, M. A., Stevenson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SC.ACC/AHA+Guidelines+for+the+Evaluation+and+Management+of+Chronic+Heart+Failure+in+the+Adult:+Executive+Summary+A+Report+of+the+American+College+of+Cardiology/American+Heart+Association+Task+Force+on+Practice+Guidelines+(Committee+to+Revise+the+1995+Guidelines+for+the+Evaluation+and+Management+of+Heart+Failure).">SC.ACC/AHA Guidelines for the Evaluation and Management of Chronic Heart Failure in the Adult: Executive Summary A Report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Committee to Revise the 1995 Guidelines for the Evaluation and Management of Heart Failure).</a> Circulation. 104, 2996-3007 (2001).</li><li>Goldman, S., Raya, T. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Universal+Definition+of+Myocardial+Infarction.">Universal Definition of Myocardial Infarction.</a> Circulation. 116, 2634-2653 (2007).</li><li>Vasilyev, N., Williams, T., Brennan, M. L., Unzek, S., Zhou, X., Heinecke, J. W., Spitz, D. R., Topol, E. J., Hazen, S. L., Penn, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myeloperoxidase-generated+oxidants+modulate+left+ventricular+remodeling+but+not+infarct+size+after+myocardial+infarction.">Myeloperoxidase-generated oxidants modulate left ventricular remodeling but not infarct size after myocardial infarction.</a> Circulation. 112, 2812-2820 (2005).</li><li>McConnell, B. K., Popovic, Z., Mal, N., Lee, K., Bautista, J., Forudi, F., Schwartzman, R., Jin, J. P., Penn, M., Bond, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Disruption+of+protein+kinase+A+interaction+with+A-kinase-anchoring+proteins+in+the+heart+in+vivo:+effects+on+cardiac+contractility,+protein+kinase+A+phosphorylation,+and+troponin+I+proteolysis.">Disruption of protein kinase A interaction with A-kinase-anchoring proteins in the heart in vivo: effects on cardiac contractility, protein kinase A phosphorylation, and troponin I proteolysis.</a> J Biol Chem. 284, 1583-1592 (2009).</li><li>Monte, D. e. l., Butler, F., Boecker, K., Gwathmey, W., K, J., Hajjar, R. 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Острый инфаркт миокарда (ОИМ) у крыс, также известный как ишемия-реперфузия (ИК), обычно используется для имитации человеческой ремоделирование желудочка приводит к сердечной недостаточности. Прогрессирование дисфункции ЛЖ и сердечной недостаточности на животных моделях (крысы и мыш?...

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acute myocardial infarction in rats

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular diseases. Animal models that mimic human cardiac disease, such as myocardial infarction (MI) and ischemia-reperfusion (IR) that induces heart failure as well as pressure-overload (transverse aortic constriction) that induces cardiac hypertrophy and heart failure (Goldman and Tarnavski), are useful models to study cardiovascular disease. In particular, myocardial ischemia (MI) is a leading cause for cardiovascular morbidity and mortality despite controlling certain risk factors such as arteriosclerosis and treatments via surgical intervention (Thygesen). Furthermore, an acute loss of the myocardium following myocardial ischemia (MI) results in increased loading conditions that induces ventricular remodeling of the infarcted border zone and the remote non-infarcted myocardium. Myocyte apoptosis, necrosis and the resultant increased hemodynamic load activate multiple biochemical intracellular signaling that initiates LV dilatation, hypertrophy, ventricular shape distortion, and collagen scar formation. This pathological remodeling and failure to normalize the increased wall stresses results in progressive dilatation, recruitment of the border zone myocardium into the scar, and eventually deterioration in myocardial contractile function (i.e. heart failure). The progression of LV dysfunction and heart failure in rats is similar to that observed in patients who sustain a large myocardial infarction, survive and subsequently develops heart failure (Goldman). The acute myocardial infarction (AMI) model in rats has been used to mimic human cardiovascular disease; specifically used to study cardiac signaling mechanisms associated with heart failure as well as to assess the contribution of therapeutic strategies for the treatment of heart failure. The method described in this report is the rat model of acute myocardial infarction (AMI). This model is also referred to as an acute ischemic cardiomyopathy or ischemia followed by reperfusion (IR); which is induced by an acute 30-minute period of ischemia by ligation of the left anterior descending artery (LAD) followed by reperfusion of the tissue by releasing the LAD ligation (Vasilyev and McConnell). This protocol will focus on assessment of the infarct size and the area-at-risk (AAR) by Evan's blue dye and triphenyl tetrazolium chloride (TTC) following 4-hours of reperfusion; additional comments toward the evaluation of cardiac function and remodeling by modifying the duration of reperfusion, is also presented. Overall, this AMI rat animal model is useful for studying the consequence of a myocardial infarction on cardiac pathophysiological and physiological function.

Watch this Scientific Journal Video about Acute Myocardial Infarction in Rats at JoVE.com

Acute Myocardial Infarction in Rats

Крысиной модели острого инфаркта миокарда (ОИМ) полезен для изучения последствий МИ на сердечную патофизиологические и физиологические функции.

Острого инфаркта миокарда у крыс

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

Research

JoVE Journal

Medicine

Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury

Индукции инфаркта миокарда у взрослых данио рерио использование Cryoinjury

The mammalian heart is incapable of significant regeneration following an acute injury such as myocardial infarction1. By contrast, urodele amphibians and ...

MRI and PET in Mouse Models of Myocardial Infarction

МРТ и ПЭТ в мыши модели инфаркта миокарда

Myocardial infarction is one of the leading causes of death in the Western world. The similarity of the mouse heart to the human heart has made it an ...

Myocardial Infarction and Functional Outcome Assessment in Pigs

Инфаркт миокарда и функциональная оценка результат в Свиней

Introduction of newly discovered cardiovascular therapeutics into first-in-man trials depends on a strictly regulated ethical and legal roadmap. One ...

Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

Первичная оценка Результат в Pig модели острого инфаркта миокарда

Mortality after acute myocardial infarction remains substantial and is associated with significant morbidity, like heart failure. Novel therapeutics are ...

Myocardial Infarction in Neonatal Mice, A Model of Cardiac Regeneration

Инфаркт миокарда у новорожденных мышей, Модель регенерации сердца

Myocardial infarction induced by coronary artery ligation has been used in many animal models as a tool to study the mechanisms of cardiac repair and ...

Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice

Анализ реального времени давления объем острого инфаркта миокарда у мышей

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any ...

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Цифровая ПЦР для количественного определения циркулирующих микроРНК в острый инфаркт миокарда и сердечно-сосудистых заболеваний

Circulating serum microRNAs (miRNAs) have shown promise as biomarkers for the cardiovascular disease and acute myocardial infarction (AMI), being released ...

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

Криотравма модель для изучения инфаркта миокарда в мыши

The use of animal models is essential for developing new therapeutic strategies for acute coronary syndrome and its complications. In this article, we ...

Semi-Minimal Invasive Method to Induce Myocardial Infarction in Rats and the Assessment of Cardiac Function by an Isolated Working Heart System

Полумапримальная инвазивная методика для индуцирования инфаркта миокарда у крыс и оценка сердечной функции изолированной рабочей системой сердца

Myocardial infarction (MI) remains the main contributor to morbidity and mortality worldwide. Therefore, research on this topic is mandatory. An easily ...

Utilizing Percutaneous Ventricular Assist Devices in Acute Myocardial Infarction Complicated by Cardiogenic Shock

Использование чрескожных желудочковых вспомогательных устройств при остром инфаркте миокарда, осложненном кардиогенным шоком

Cardiogenic shock is defined as persistent hypotension, accompanied by evidence of end organ hypo-perfusion. Percutaneous ventricular assist devices ...