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DOI :
10.3791/2466-v
•
10:40 min
January 7th, 2011
Chapters
0:05
Title
1:22
Introduction
2:45
Making Electrodes and Set Up of Electroporation Station
4:09
Microinjection of Morpholinos into the Zebrafish Otic Vesicle
6:52
Electroporation Procedure
8:13
Representative Ear Injection Results
10:21
Conclusion
24hpfでゼブラフィッシュの耳胞に直接モルフォを提供する方法が開発されている。耳胞と浸透をもたらすためにエレクトロポレーションの内腔にモルフォのマイクロインジェクションを使用して、我々は脳にモルフォのエフェクトをバイパスし、内耳に固有の効果を得ることができた。
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