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DOI :
10.3791/2468-v
•
8:53 min
February 17th, 2011
Chapters
0:05
Title
2:01
Preparation of Cell Line with an Integrated Recombination Substrate
3:04
Transfection to Deplete Endogenous BRCA1 and add back Mutant BRCA1
5:19
Analysis of Transfectants
6:07
Analysis of the Effects of BRCA1 Mutations on Homologous Recombination via GFP-Expression
7:54
Conclusion
我们提供了一个用于测试在一个组织文化同源重组修复DNA损伤的检测消耗使用RNA干扰细胞内源性BRCA1蛋白和替换它与BRCA1基因点突变,其中包含一个编码变化的BRCA1基因变体的方法。
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